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Sigma-Aldrich

Protein A, Biotin Conjugate

This Protein A, Biotin Conjugate is validated for use in Solid Phase Immunoassay.

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Quality Level

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

ambient

storage temp.

−20°C

General description

Native protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.
Native protein A conjugated to biotin.

Application

Solid Phase Immunoassay (1:10,000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, 1% BSA, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

A single band at MW 50,000 by SDS-PAGE

Other Notes

The protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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