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P2165

Sigma-Aldrich

Protein A–Biotin from Staphylococcus aureus

lyophilized powder

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MDL number:
NACRES:
NA.46

conjugate

biotin conjugate

Quality Level

200
300

form

lyophilized powder

composition

Protein, ≥70% biuret

extent of labeling

3.0-8.0 mol biotin per mol Protein A

storage temp.

2-8°C

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This Item
P3838P8651H9395
storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

-

Quality Level

200, 300

Quality Level

200

Quality Level

100, 200

Quality Level

300

composition

Protein, ≥70% biuret

composition

-

composition

-

composition

Protein, ~60% Lowry

extent of labeling

3.0-8.0 mol biotin per mol Protein A

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Protein A (SpA) is a 42kDa virulence factor produced by Staphylococcus aureus, and is composed of five highly homologous consecutive extracellular Ig-binding domains, namely domains E, D, A, B, and C. This protein is present in both secreted and membrane-associated forms.

Specificity

Binds IgG only from most mammals, except rat, goat, and sheep

Biochem/physiol Actions

Protein A (SpA) causes B cells death in vitro, by binding to the Fc region of antibody and the Fab regions of the B-cell receptor, thus inhibiting its opsonophagocytosis. This protein is thought to be implicated in the evasion of host immune responses by Staphylococcus aureus, and thus, might be a candidate for vaccine development approaches to prevent severe S. aureus infections. S. aureus is responsible for nosocomial infections as well as a variety of human infections, such as food poisoning, toxic shock syndrome, endocarditis, septicemia, skin infections, soft tissue infections, and bone infections. It is also responsible for bovine and ovine mastitis. SpA typing or DNA sequence analysis of the variable repeat region of protein A gene is useful in determining S. aureus outbreak strains from those strains that are epidemiologically insignificant.

Physical form

Lyophilized powder containing sodium citrate

Preparation Note

Prepared from P 6031, coupled to biotin by an amide bond through an aminocaproyl spacer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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M Balasch et al.
Journal of comparative pathology, 121(2), 139-148 (1999-07-16)
This report describes the experimental inoculation of conventional pigs with a tissue homogenate obtained from two pigs affected with postweaning multisystemic wasting syndrome (PMWS). Eight 2-month-old pigs were inoculated by the intranasal route, and two pigs were left as uninfected
Anil K Pal et al.
Biosensors, 10(10) (2020-09-30)
We report a novel self-referenced diffraction-based leaky waveguide (LW) comprising a thin (~2 µm) film of a photofunctionalisable hydrogel created by covalent attachment of a biotinylated photocleavable linker to chitosan. Streptavidin attached to the chitosan via the photocleavable linker was
spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation.
Koreen L et al
Journal of Clinical Microbiology, 42(2), 792-799 (2004)
Regina Stoltenburg et al.
International journal of molecular sciences, 19(2) (2018-03-03)
New, as yet undiscovered aptamers for Protein A were identified by applying next generation sequencing (NGS) to a previously selected aptamer pool. This pool was obtained in a classical SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiment using the
Scott D Kobayashi et al.
mBio, 4(5), e00764-e00713 (2013-10-03)
Staphylococcus aureus is a prominent cause of human infections worldwide and is notorious for its ability to acquire resistance to antibiotics. Methicillin-resistant S. aureus (MRSA), in particular, is endemic in hospitals and is the most frequent cause of community-associated bacterial

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