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F0504

Sigma-Aldrich

Folate binding protein from bovine milk

lyophilized powder

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MDL number:
NACRES:
NA.61

biological source

bovine milk

Quality Level

form

lyophilized powder

usage

 mg sufficient for ~4,000 assays

composition

Protein, 20-40% modified Warburg-Christian

technique(s)

ligand binding assay: suitable
surface plasmon resonance (SPR): suitable

capacity

1.5-6.0 μg/mg, protein binding capacity (folic acid)(25 °C at pH 7.5.)

storage temp.

−20°C

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Application

Folate binding protein can be used in radioligand binding assays of serum folate. The folate receptor has been used in studies as a therapeutic target in a variety of cancers.
Folate binding protein from bovine milk has been used in folate binding studies using surface plasmon resonance and in the uptake studies in kidney proximal tubule cells.
Useful for radioligand binding assays of serum folate. May also bind N-methyltetrahydrofolic acid and other folate analogues.

Biochem/physiol Actions

Folate-binding protein (FBP) is present as a cell receptor and binds to folate in equimolar ratio. It is involved in the transport of folate and improves their bioavailability.

Packaging

Package size based on protein content

Quality

Purified over 1,000 fold. May contain a trace of β-mercaptoethanol.

Physical form

Lyophilized powder containing buffer salt as sodium citrate

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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