P5511
Protein Kinase A from bovine heart
≥0.8 units/μg protein, lyophilized powder
Synonym(s):
Protein Kinase, 3′,5′-cyclic-AMP-dependent from bovine heart
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.77
form
lyophilized powder
Quality Level
specific activity
≥0.8 units/μg protein
composition
Protein, ≥70%
storage temp.
−20°C
Gene Information
cow ... PRKACA(282322), PRKACB(282323)
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General description
Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme.
Application
Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. It has also been used to study the interaction of staurosporine with the ATP-binding site of kinases.
Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin.
Biochem/physiol Actions
Many 3′,5′-cyclic AMP dependent protein kinases have been reported. Structural studies (Traugh, J.A., et al., Meth. Enzymol., 38, 290 [1974]) show the presence of at least two subunits, the regulatory subunit and the catalytic subunit. When both units are linked together, the catalytic activity is inhibited. However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins.
Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. It regulates apoptosis, mitochondrial respiration and ATP synthesis. PKA is modulated by protease calpain.
Packaging
Package size based on protein content
Physical form
Crude lyophilized powder containing EDTA and potassium phosphate, pH 7.5.
Preparation Note
Fractionated essentially by procedure of Gilman, A., Proc. Natl. Acad. Sci. USA, 67, 305 (1970).
Analysis Note
Protein determined by biuret.
Other Notes
One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C in the presence of cyclic AMP, determined by measuring the production of ADP.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Probes of the mitochondrial cAMP-dependent protein kinase
Shell JR and Lawrence DS
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1834(7), 1359-1363 (2013)
Nitroglycerine-and isoprenaline-induced vasodilatation: assessment from the actions of cyclic nucleotides
Itohy T, et al.
British Journal of Pharmacology, 84(2), 393-406 (1985)
Y Ito et al.
British journal of pharmacology, 83(3), 677-686 (1984-11-01)
Effects of isoprenaline (Isop) on the contractile properties of the smooth muscle cells of cat trachea were investigated using intact and chemically skinned muscle preparations and an isometric tension recording method. In the intact muscle preparations, Isop 3 X 10(-10)
Péter Enyedi et al.
PloS one, 9(5), e97854-e97854 (2014-05-17)
The cytoplasmic loop between the second and third transmembrane segments is pivotal in the regulation of TRESK (TWIK-related spinal cord K+ channel, K2P18.1, KCNK18). Calcineurin binds to this region and activates the channel by dephosphorylation in response to the calcium
Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe
Journal of Bioenergetics and Biomembranes (2017)
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