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204860

Sigma-Aldrich

Complement C3b, Human

Complement C3b, Human, is a native C3b complement component. It is a glycoprotein composed of the modified C3-α chain (αʹ) (M.W. 105,000) and the intact C3-β chain (M.W. 75,000).

Synonym(s):

C3b, Complement C3

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.25

Quality Level

assay

≥90% (SDS-PAGE)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

impurities

IgG, IgA, IgM, C5, Factor H, or Factor I, trace

shipped in

wet ice

storage temp.

−70°C

General description

Native, human C3b complement component. Cleavage of the C3-α chain at peptide bond 77 by either of the complement C3 convertase enzymes results in production of C3a (M.W. 9083) and C3b (M.W. 180,000) fragments. The C3b fragment is a glycoprotein composed of the modified C3-α chain (α′) (M.W. 105,000) and the intact C3-β chain (M.W. 75,000). Nascent C3b has the transient ability to form a covalent ester bond with a variety of target surfaces. Once bound to target surfaces C3b becomes an essential subunit of both the classical and alternative pathway C5 cleaving enzymes. In addition, surface-bound C3b has opsonic and immune adherence activities which are mediated via binding to CR1 (CD35) complement receptors.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.2, filtered through a 0.22 µm filter.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Wong, W.W. and Fearon, D.T. 1987. Methods Enzymol.150, 579.
Arnout, M.A., et al. 1981. J. Immunol.127, 1348.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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