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Sigma-Aldrich

Caspase-3 Substrate II, Fluorogenic

Fluorogenic caspase-3 substrate.

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Synonym(s):
Caspase-3 Substrate II, Fluorogenic, Ac-DEVD-AMC
Empirical Formula (Hill Notation):
C30H37N5O13
CAS Number:
Molecular Weight:
675.64
MDL number:

Quality Level

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

off-white

solubility

DMSO: 10 mM

fluorescence

λex 365-380 nm
λem 430-460 nm

shipped in

ambient

storage temp.

−20°C

InChI

1S/C30H37N5O13/c1-13(2)26(35-27(44)18(7-8-22(37)38)33-29(46)19(11-23(39)40)31-15(4)36)30(47)34-20(12-24(41)42)28(45)32-16-5-6-17-14(3)9-25(43)48-21(17)10-16/h5-6,9-10,13,18-20,26H,7-8,11-12H2,1-4H3,(H,31,36)(H,32,45)(H,33,46)(H,34,47)(H,35,44)(H,37,38)(H,39,40)(H,41,42)/t18-,19-,20-,26-/m0/s1

InChI key

ALZSTTDFHZHSCA-RNVDEAKXSA-N

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This Item
264151264150218765
Sigma-Aldrich

218765

Caspase-9 Substrate I, Fluorogenic

form

solid

form

lyophilized solid

form

solid

form

solid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

DMSO: 10 mM

solubility

DMSO: 5 mg/mL

solubility

DMSO: 10 mg/mL

solubility

DMSO: 5 mg/mL

General description

Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (Km = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.
Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (Km = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no
Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3
Primary Target
Fluorogenic caspase-3 substrate
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Glu-Val-Asp-AMC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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