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264150

Sigma-Aldrich

Caspase-3 Substrate IV, Fluorogenic

Fluorogenic caspase-3 substrate.

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Synonym(s):
Caspase-3 Substrate IV, Fluorogenic, Z-DEVD-AFC
Empirical Formula (Hill Notation):
C36H38F3N5O14
CAS Number:
Molecular Weight:
821.71

Quality Level

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white to off-white

solubility

DMSO: 10 mg/mL

fluorescence

λex ~400 nm
λem ~505 nm

shipped in

ambient

storage temp.

−20°C

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This Item
264155264151218765
Caspase-3 Inhibitor II The Caspase-3 Inhibitor II, also referenced under CAS 210344-95-9, controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

264155

Caspase-3 Inhibitor II

Sigma-Aldrich

218765

Caspase-9 Substrate I, Fluorogenic

form

solid

form

solid

form

lyophilized solid

form

solid

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light

General description

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.
Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Fluorogenic caspase-3 substrate.
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Z-Asp-Glu-Val-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Kluck, R.M., et al. 1997. Science275, 1132.
Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.
Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.
Nicholson, D.W., et al. 1995. Nature376, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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