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324676

Sigma-Aldrich

Elastase, Pseudomonas aeruginosa

Elastase, Pseudomonas aeruginosa, is a native metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin.

Synonym(s):

Pseudolysin

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

assay

≥90% (SDS-PAGE)

form

lyophilized

specific activity

≥250 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

aqueous buffer: 1 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

Native elastase from Pseudomonas aeruginosa. A metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin. Pseudomonas aeruginosa elastase has been implicated in the reinforcement of the inflammation process in cases of infection with the bacterium.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µg insoluble elastin per h at 37°C.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 2 months at -20°C.

Other Notes

Azghani, A.O., et al. 2000. Lung178, 181.
Kon, Y., et al. 1999. FEMS Immunol. Med. Microbiol. 25, 313.
Braun, P., et al. 1998. J. Bacteriol. 180, 3467.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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