324713
Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus
Synonym(s):
Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus, V8 Protease
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Pricing and availability is not currently available.
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biological source
Staphylococcus aureus
Quality Level
grade
Proteomics Grade
form
lyophilized
specific activity
≥10 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
ambient
storage temp.
2-8°C
Related Categories
General description
Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Warning
Toxicity: Harmful (C)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Other Notes
Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA69, 3506.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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S Zhang et al.
The Journal of biological chemistry, 271(52), 33575-33579 (1996-12-27)
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Victoria L Stefanelli et al.
Matrix biology : journal of the International Society for Matrix Biology, 82, 86-104 (2019-04-21)
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