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ENDOARGS-RO

Roche

Endoproteinase Asp-N Sequencing Grade

from Pseudomonas fragi

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Synonym(s):
protease
Enzyme Commission number:

Assay

≥90% (SDS-PAGE)

form

lyophilized

specific activity

≥20000 units/mg protein

mol wt

27 kDa

packaging

pkg of 2 μg (11420488001)
pkg of 3 × 2 μg (11054589001)

manufacturer/tradename

Roche

optimum pH

7.0-8.0

pH range

6.5-9.0

storage temp.

2-8°C

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Trypsin Sequencing Grade from bovine pancreas

Roche

TRYPSEQ-RO

Trypsin Sequencing Grade

form

lyophilized

form

lyophilized (salt-free)

form

lyophilized

form

lyophilized (salt-free)

specific activity

≥20000 units/mg protein

specific activity

≥15 units/mg protein

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

specific activity

≥80 units/mg protein

mol wt

27 kDa

mol wt

Mr 27 kDa

mol wt

33 kDa by SDS-PAGE (reducing)

mol wt

Mr 23.5 kDa

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

optimum pH

7.0-8.0

optimum pH

4.0-7.8

optimum pH

8.5-8.8

optimum pH

8.0

General description

The wild type of Pseudomonas fragi segregates a metalloprotease, which cleaves peptide bonds N-terminally at small hydrophilic amino acids. A mutant of this strain produces the Endoproteinase Asp-N Sequencing Grade, which is isolated as a highly purified and specific protease.

Specificity

Endoproteinase Asp-N Sequencing Grade is a metalloprotease. In phosphate, acetate, or Tris buffers at pH 6.0–8.5, it specifically cleaves peptide bonds N-terminally at aspartic and cysteic acid. If cysteine is reduced or alkylated, only -↓-Asp-X is cleaved.
The specificity of Endoproteinase Asp-N Sequencing Grade is verified with glucagon as a substrate. High concentrations of Endoproteinase Asp-N Sequencing Grade (1 part by weight enzyme with 10 parts by weight glucagon) are incubated to detect traces of impurities like contaminating proteases.
Heat inactivation: The protease is stopped by boiling for 5 minutes.

Application

Use Endoproteinase Asp-N Sequencing Grade for protein structure analysis and sequence analysis.

Preparation Note

Working concentration: 2 μg /50 μl double-dist. water
One vial (= 2 μg enzyme) is dissolved in 50 μl double-dist. water resulting in a buffer concentration of 10 mM Tris-HCl pH 7.5.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): -15 to -25 °C
A solution in double-dist. water is stable for 1 week at maximum at 2 to 8 °C and for about 1 month at -15 to -25 °C; repeated thawing should be avoided.

Reconstitution

Lyophilized Endoproteinase Asp-N sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 10 mM Tris-HCl, pH 7.5. In order to avoid autolysis the incubation temperature should not exceed 37 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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