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ENDOGLUS-RO

Roche

Endoproteinase Glu-C Sequencing Grade

from Staphylococcus aureus V8

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Synonym(s):
protease
Enzyme Commission number:

biological source

Staphylococcus aureus (V8)

form

lyophilized (salt-free)
lyophilized

specific activity

≥15 units/mg protein

mol wt

Mr 27 kDa

packaging

pkg of 3 × 50 μg (11047817001)
pkg of 50 μg (11420399001)

manufacturer/tradename

Roche

optimum pH

4.0-7.8

storage temp.

2-8°C

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ENDOLYSS-RO10791156001TRYPSEQ-RO
Trypsin Sequencing Grade from bovine pancreas

Roche

TRYPSEQ-RO

Trypsin Sequencing Grade

form

lyophilized (salt-free)

form

lyophilized

form

lyophilized (salt-free)

form

lyophilized (salt-free)

specific activity

≥15 units/mg protein

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

specific activity

20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).), ~20 units/mg protein (At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).)

specific activity

≥80 units/mg protein

mol wt

Mr 27 kDa

mol wt

33 kDa by SDS-PAGE (reducing)

mol wt

30 kDa

mol wt

Mr 23.5 kDa

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

optimum pH

4.0-7.8

optimum pH

8.5-8.8

optimum pH

8.0-8.5

optimum pH

8.0

General description

Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.

Specificity

Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.

Application

Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.

Features and Benefits

Free of impurities that may interfere with separation of peptides in RP HPLC.

Preparation Note

Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.

Reconstitution

Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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Daniel Kolarich et al.
Nature protocols, 7(7), 1285-1298 (2012-06-09)
The comprehensive analysis of protein glycosylation is a major requirement for understanding glycoprotein function in biological systems, and is a prerequisite for producing recombinant glycoprotein therapeutics. This protocol describes workflows for the characterization of glycopeptides and their site-specific heterogeneity, showing
Chen Li et al.
mAbs, 5(4), 565-575 (2013-06-12)
In developing biosimilar or biobetter products, comparability to the reference product is required to claim similar integrity or intended purpose. In this work, an anti-CD20 monoclonal antibody developed using RNA interference to decrease core fucosylation (RNAi-mediated) was comprehensively characterized by

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