341578

Fibrinogen, Plasminogen-Depleted, Human Plasma

Plasminogen-Depleted fibrinogen is a native fibrinogen from which plasminogen is depleted using a Lys-agarose column. Contains ≥ 95% clottable proteins.

• Synonym(s): Fibrinogen, Plasminogen-Depleted, Human Plasma
• CAS Number: 9001-32-5
• MDL number: MFCD00012459
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• biological source: human plasma
• Quality Level: 200
• description: Merck USA index - 14, 4071
• assay: ≥95% (Clottable proteins)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: 25 mg/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Native fibrinogen from human plasma, depleted of plasminogen using a Lys-agarose column. An acute-phase plasma protein found at about 300 mg/dl. Conversion of soluble fibrinogen to the insoluble clot-forming fibrin is the terminal stage of blood clotting. Clinically, fibrinogen is found at elevated levels during pregnancy, after surgery trauma, or in cases of myocardial infarction. It is found at decreased levels in cases of liver cirrhosis and serum hepatitis. The plasminogen was depleted using a Lys-agarose column.

Warning

Toxicity: Harmful (C)

Preparation Note

To prevent clot formation, warm the H₂O and the fibrinogen to 37°C prior to dissolution.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or store at room temperature (20°C) for short-term storage. Stock solutions are stable for up to 4 h at 20°C or for up to 6 months at -70°C. Do not store solutions on ice; precipitation will occur. Stock solutions stored at -70°C should be thawed in a water bath maintained at 37°C.

Analysis Note

homogeneous by SDS-PAGE

Other Notes

Wang, H., et al. 1995. J. Infect. Dis.171, 85.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3