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484235

Sigma-Aldrich

p-Nitroblue Tetrazolium Chloride

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC₅₀ = 3-4 µM).

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Synonym(s):
p-Nitroblue Tetrazolium Chloride, NBT, Nitro BT
Empirical Formula (Hill Notation):
C40H30Cl2N10O6
CAS Number:
Molecular Weight:
817.64
MDL number:

Quality Level

assay

≥98% (TLC)

form

crystalline solid

potency

3-4 μM IC50

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

yellow

solubility

methanol: soluble
water: soluble

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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This Item
N6876N5514N6639
p-Nitroblue Tetrazolium Chloride NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC₅₀ = 3-4 µM).

484235

p-Nitroblue Tetrazolium Chloride

Nitrotetrazolium Blue chloride ≥90.0% (HPLC)

N6876

Nitrotetrazolium Blue chloride

Nitro Blue Tetrazolium tablet

N5514

Nitro Blue Tetrazolium

Nitrotetrazolium Blue chloride powder, electrophoresis grade

N6639

Nitrotetrazolium Blue chloride

assay

≥98% (TLC)

assay

≥90.0% (HPLC)

assay

-

assay

>90%

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

methanol: soluble, water: soluble

solubility

H2O: 10 mg/mL

solubility

soluble, clear, yellow

solubility

H2O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.), 2-methoxyethanol: 20 mg/mL, ethanol: 5 mg/mL

General description

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).

Biochem/physiol Actions

Primary Target
NOS

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.

Other Notes

Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Jian-Xin Diao et al.
Chinese journal of integrative medicine, 26(10), 736-744 (2019-11-27)
To investigate the phenolic composition, antioxidant properties, and hepatoprotective mechanisms of polyphenols from green tea extract (GTP) in carbon tetrachloride (CCl4)-induced acute liver injury mouse model. High-performance liquid chromatography was used to analyze the chemical composition of the extract. Antioxidant

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