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11383213001

Roche

NBT

4-Nitro blue tetrazolium chloride, solution

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Synonym(s):
Nitroblue tetrazolium chloride

Quality Level

assay

>95%

form

solution

mol wt

Mr 817.7

packaging

pkg of 3 mL (300 mg)

manufacturer/tradename

Roche

technique(s)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

storage temp.

−20°C

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This Item
SRP45151168561991011175041910
vibrant-m

11383213001

NBT

vibrant-m

SRP4515

Artemin human

vibrant-m

11685619910

Fluorescein RNA Labeling Mix

assay

>95%

assay

≥98% (SDS-PAGE)

assay

-

assay

-

technique(s)

Northern blotting: suitable, hybridization: suitable, Southern blotting: suitable

technique(s)

-

technique(s)

Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable

technique(s)

Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

solution

form

lyophilized

form

solution

form

-

mol wt

Mr 817.7

mol wt

~24 kDa

mol wt

-

mol wt

-

General description

NBT is a chromogenic and soluble substance. It is a substrate of alkaline phosphatase (AP) and is converted to an indigo/purple dye on its action.

Application

  • Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

Physical form

Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used.

Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.

Preparation Note

Working solution: Preparation of staining solution
  • Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
  • Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2

flash_point_f

167.0 °F

flash_point_c

75 °C


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Protocols

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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