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B6149

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl phosphate disodium salt

≥98% (HPLC)

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Synonym(s):
5-BCIP disodium salt, BCIP®, X-phosphate disodium salt
Linear Formula:
C8H4BrClNO4P · 2Na
CAS Number:
Molecular Weight:
370.43
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

assay

≥98% (HPLC)

form

powder

solubility

H2O: 20 mg/mL
DMF: insoluble

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12

InChI

1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2

InChI key

OAZUOCJOEUNDEK-UHFFFAOYSA-L

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This Item
B6777I5505B8503
Indoxyl phosphate disodium salt crystalline

I5505

Indoxyl phosphate disodium salt

solubility

H2O: 20 mg/mL, DMF: insoluble

solubility

DMF: 20 mg/mL, H2O: insoluble

solubility

water: 5 mg/mL, clear to slightly hazy

solubility

DMF: 20 mg/mL, clear to slightly hazy, yellow

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

crystalline

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Substrates

Histochemical substrate for alkaline phosphatase.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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