Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

11383221001

Roche

BCIP

solution, >95% (HPLC), pkg of 3 mL (150 mg)

Synonym(s):

4-toluidine salt

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204

description

4-toluidine salt

Quality Level

assay

>95% (HPLC)

form

solution

packaging

pkg of 3 mL (150 mg)

manufacturer/tradename

Roche

shipped in

dry ice

storage temp.

−20°C

General description

BCIP serves as a substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

Use BCIP in alkaline phosphatase detection:
  • Southern blot
  • Northern blot
  • Western blot
  • Colony and plaque hybridization
  • In situ hybridization

Quality

Function test: incubation with AP

Principle

Use of BCIP as substrate for alkaline phosphatase in combination with nitro blue tetrazolium chloride (NBT) as electron acceptor: Substrates and reaction products of alkaline phosphatase catalyzed color reaction with NBT/BCIP.

Physical form

Solution (50 mg/ml) in dimethylformamide (DMF)

Other Notes

For life science research only. Not for use in diagnostic procedures.

signalword

Danger

Hazard Classifications

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Repr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zoe Crane-Smith et al.
Frontiers in cell and developmental biology, 9, 595744-595744 (2021-04-20)
Enhancers that are conserved deep in evolutionary time regulate characteristics held in common across taxonomic classes. Here, deletion of the highly conserved Shh enhancer SBE2 (Shh brain enhancer 2) in mouse markedly reduced Shh expression within the embryonic brain specifically
Jinlong Chen et al.
Dental materials : official publication of the Academy of Dental Materials, 36(10), 1303-1313 (2020-07-12)
Compared to autologous bone grafts, allogeneic bone grafts integrate slowly, which can adversely affect clinical outcomes. Here, our goal was to understand the molecular mechanisms underlying graft incorporation, and then test clinically feasible methods to accelerate this process. Wild-type and
Nicoletta I Petridou et al.
Cell, 184(7), 1914-1928 (2021-03-18)
Embryo morphogenesis is impacted by dynamic changes in tissue material properties, which have been proposed to occur via processes akin to phase transitions (PTs). Here, we show that rigidity percolation provides a simple and robust theoretical framework to predict material/structural
Surendra Singh Patel et al.
The Journal of experimental biology, 223(Pt 8) (2020-02-27)
Eisenia fetida, the common vermicomposting earthworm, shows robust regeneration of posterior segments removed by amputation. During the period of regeneration, the newly formed tissue initially contains only undifferentiated cells but subsequently differentiates into a variety of cell types including muscle
Ryuma Haraguchi et al.
International journal of molecular sciences, 22(3) (2021-02-04)
The reproductive tract in mammals emerges from two ductal systems during embryogenesis: Wolffian ducts (WDs) and Mullerian ducts (MDs). Most of the female reproductive tract (FRT) including the oviducts, uterine horn and cervix, originate from MDs. It is widely accepted

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service