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BCIPP-RO

Roche

BCIP

99% (HPLC), powder, solubility: in DMF, soluble

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Synonym(s):
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt
Empirical Formula (Hill Notation):
C8H6BrClNO4P · C7H9N
CAS Number:
Molecular Weight:
433.62
MDL number:
PubChem Substance ID:

description

5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt

Quality Level

assay

99% (HPLC)

form

powder

mol wt

Mr 326.44 (BCIP)
Mr 433.6 (BCIP toluidine salt)

packaging

pkg of 1 g (11585002001)
pkg of 250 mg (10760994001)

manufacturer/tradename

Roche

solubility

DMF: soluble

storage temp.

2-8°C

SMILES string

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI key

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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This Item
B8503B677711383221001
vibrant-m

BCIPP-RO

BCIP

vibrant-m

11383221001

BCIP

solubility

DMF: soluble

solubility

DMF: 20 mg/mL, clear to slightly hazy, yellow

solubility

DMF: 20 mg/mL, H2O: insoluble

solubility

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

powder

form

powder

form

powder

form

solution

description

5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt

description

-

description

-

description

4-toluidine salt

General description

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Quality

Performance test: incubation with AP

Substrates

Histochemical substrate for alkaline phosphatase.

Analysis Note

Absorption: Absorption maximum in the visible light is not determined.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

136.4 °F

flash_point_c

58 °C


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