5.06029

LipidGreen2

• Synonym(s): LipidGreen2
• Empirical Formula (Hill Notation): C₂₂H₂₉NO₄
• Molecular Weight: 371.47
• UNSPSC Code: 12352202
• NACRES: NA.21

Properties

• assay: ≥97% (HPLC)
• Quality Level: 100
• form: oil
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: orange-yellow
• solubility: DMSO: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable N-dimethylallylated 3-hydroxyindole based compound that is shown to selectively stain neutral lipids, but not phospholipids, in various cells lines with bright fluorescence and with minimal non-specific background. Generates stronger staining with lipid droplets (~10 µM) when compared to Nile Red. Shown to be more effective than BODIPY^® 493/503 in assessing hepatotoxicity of several known compounds using HepG2 cells. Also suitable for staining fat deposits in developing zebrafish embryos. Can be used in conjunction with LipidToxt red dye for multicolor imaging of neutral lipids.

A cell-permeable N-dimethylallylated 3-hydroxyindole based compound that is shown to selectively stain neutral lipids, but not phospholipids, in various cells lines with bright fluorescence and with minimal non-specific background. Generates stronger staining with lipid droplets (~10 µM) when compared to Nile Red. Shown to be more effective than BODIPY^® 493/503 in assessing hepatotoxicity of several known compounds using HepG2 cells. Also suitable for staining fat deposits in developing zebrafish embryos. Can be used in conjunction with LipidToxt red dye for multicolor imaging of neutral lipids.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Biochem/physiol Actions

Cell permeable: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Chun, H., et al, 2013, Mol Biosys.9, 630.

Legal Information

BODIPY is a registered trademark of Life Technologies

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable