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Pronase® Protease, Streptomyces griseus, Nuclease-Free

Widely used broad-spectrum protease mixture

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Synonym(s):
Nuclease-Free Protease, Streptomyces griseus, Pronase® Protease, Pronase® Protease, Streptomyces griseus
CAS Number:
MDL number:

form

lyophilized

specific activity

≥70,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

water: 10 mg/mL

foreign activity

Nuclease, none detected

shipped in

ambient

storage temp.

2-8°C

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This Item
537027433P5147
vibrant-m

7433

Pronase E

vibrant-m

P5147

Protease from Streptomyces griseus

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

wet ice

storage condition

OK to freeze

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze

storage condition

-

form

lyophilized

form

lyophilized

form

fine powder

form

powder

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

solubility

water: 10 mg/mL

solubility

water: 10 mg/mL

solubility

-

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble 0.2 mg/mL at 37 °C, clear, colorless to tan

General description

PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

Application

Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
  • in single-cell preparations for cardiac side population (CSP) analysis
  • in aflatoxin plasma biomarker assay
  • to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

Biochem/physiol Actions

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Reconstitution

Unstable in solution; reconstitute just prior to use.

Other Notes

Note: 1 KU = 1000 units.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation markHealth hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


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Ehsan Pourkarimi et al.
eLife, 5 (2016-12-24)
The primary task of developing embryos is genome replication, yet how DNA replication is integrated with the profound cellular changes that occur through development is largely unknown. Using an approach to map DNA replication at high resolution in C. elegans
Mustafa Mahfuz et al.
Journal of exposure science & environmental epidemiology, 29(5), 655-662 (2018-09-07)
Dietary exposure to aflatoxin is implicated in growth faltering of children. Despite the high burden of childhood stunting in urban Bangladesh, there are no data on long-term exposure to aflatoxin. This study aimed to explore aflatoxin exposure levels in a
Industrial Biotechnology and Commodity Products
Comprehensive Biotechnology, 3, 587-603 null
Annette Meeson et al.
Stem cell reviews and reports, 9(5), 731-739 (2013-04-27)
Cardiac side population (CSP) cells, defined by their ability to efflux the vital dye Hoechst 33342, have been identified as putative cardiac stem cells based on their potential to give rise to both cardiomyocytes and endothelial cells. The CSP phenotype

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