539053-5T
Protein Molecular Weight Markers, HPLC
Synonym(s):
Molecular weight standards, Protein markers
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About This Item
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form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
shipped in
wet ice
storage temp.
−20°C
General description
Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW column after reconstituting with elution buffer and monitored at 280 nm. Markers include: cytochrome c, 12,400; myokinase, 32,000; enolase, 67,000; lactate dehydrogenase, 142,000; and glutamate dehydrogenase, 290,000.
Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW* column after reconstituting with elution buffer and monitored at 280 nm.
Each vial contains sufficient material for approximately 5–10 tests. There is 200 µg of each protein per vial x 5 proteins = 1000 µg total protein per vial. Each vial also contains ~5 mg sucrose as a carrier.
*Toyosoda Industry Co., Ltd.
Each vial contains sufficient material for approximately 5–10 tests. There is 200 µg of each protein per vial x 5 proteins = 1000 µg total protein per vial. Each vial also contains ~5 mg sucrose as a carrier.
*Toyosoda Industry Co., Ltd.
Note: 5T = 5 Tests.
Warning
Toxicity: Standard Handling (A)
Reconstitution
Reconstitute with 100 µl of 100 mM sodium phosphate buffer, pH 7.0 containing 200 mM NaCl. If the material is reconstituted with lower ionic strength buffer (i.e., <0.1 M NaCl) the cytochrome c may interact nonspecifically with the silica gel column and migrate incorrectly. Apply 20 µl to an analytical grade column such as TSK-Gel G3000SW (0.75 x 60 cm).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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