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Key Documents

539053-5T

Millipore

Protein Molecular Weight Markers, HPLC

Synonym(s):

Molecular weight standards, Protein markers

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About This Item

UNSPSC Code:
41105337
NACRES:
NC.01

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

General description

Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW column after reconstituting with elution buffer and monitored at 280 nm. Markers include: cytochrome c, 12,400; myokinase, 32,000; enolase, 67,000; lactate dehydrogenase, 142,000; and glutamate dehydrogenase, 290,000.
Contains five highly purified proteins ranging in molecular weight from 12.4 kDa to 290 kDa. Formulated to give peaks of approximately the same intensity for each protein when run on a TSK-Gel G300SW* column after reconstituting with elution buffer and monitored at 280 nm.



Each vial contains sufficient material for approximately 5–10 tests. There is 200 µg of each protein per vial x 5 proteins = 1000 µg total protein per vial. Each vial also contains ~5 mg sucrose as a carrier.



*Toyosoda Industry Co., Ltd.
Note: 5T = 5 Tests.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Reconstitute with 100 µl of 100 mM sodium phosphate buffer, pH 7.0 containing 200 mM NaCl. If the material is reconstituted with lower ionic strength buffer (i.e., <0.1 M NaCl) the cytochrome c may interact nonspecifically with the silica gel column and migrate incorrectly. Apply 20 µl to an analytical grade column such as TSK-Gel G3000SW (0.75 x 60 cm).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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