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Sigma-Aldrich

Anti-Synapsin I Rabbit pAb

lyophilized, Calbiochem®

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biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human, rat, mouse, bovine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Syn1(20964)

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This Item
S193324390IM1014
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

serum

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

lyophilized

form

lyophilized powder

form

lyophilized

form

liquid

species reactivity

human, rat, mouse, bovine

species reactivity

rat, mouse, human, bovine

species reactivity

human, mouse, rat

species reactivity

human

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the synapsin I protein.
Recognizes the synapsin I protein in rat brain.
This Anti-Synapsin I Rabbit pAb is validated for use in ELISA, Immunoblotting Immunocytochemistry Immunoprecipitation for the detection of Synapsin I.

Immunogen

Bovine
purified, brovine brain synapsin I

Application

ELISA (1:2500-1:10,000)

Frozen Sections (see application references)

Immunoblotting (1:200)

Immunocytochemistry (1:500-1:2500)

Immunoprecipitation (1 µg/200 µg lysate)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 5 mM ammonium bicarbonate.

Reconstitution

Reconstitute with 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat brain

Other Notes

Stone, L.M., et al. 1994. J. Neurosci.14, 301.
Mandell, J.W., et al. 1992. J. Neurosci.12, 1736.
Fletcher, T.L., et al. 1991. J. Neurosci.11, 1617.
Moore, R.Y., and Berstein, M. 1989. J. Neurosci.9, 2151.
This antibody is specific for synapsin I and can be used to estimate synaptic density and synaptogenesis. Strong and specific immunolabeling of the synapsin I doublet seen in immunoblotting at a 1:200 dilution. This immunolabeling can be blocked by preadsorption of antibody with synapsin I. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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