596105
THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound
Synonym(s):
THIOLYTE® MB Reagent, MBB
Sign Into View Organizational & Contract Pricing
Select a Size
All Photos(1)
Select a Size
Change View
About This Item
Recommended Products
assay
≥95% (HPLC)
Quality Level
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
color
yellow
solubility
acetonitrile: 13 mg/mL
methanol: 25 mg/mL
shipped in
ambient
storage temp.
10-30°C
SMILES string
BrCc1[n]2[n]([c](c1C)=O)[c](c(c2C)C)=O
Related Categories
General description
Contains a reactive functional group (allylic bromide) that is capable of reacting with a wide variety of nucleophiles to form stable covalent adducts. Reacts extremely rapidly with thiol groups under physiological conditions to yield highly fluorescent products. A labeling reaction is typically carried out at room temperature for 2 to 60 min, pH 7.4, using an equivalent amount (or a small excess) of reagent relative to the amount of thiol. Material is chemically and photolytically stable and shows an absorption maxima at 370 to 385 nm and emits light at 477 to 484 nm (turquoise). Readily penetrates membranes. Useful as an alternative tool in labeling methodology, complementing the use of such well known fluorescent labeling reagents as fluorescein and rhodamine isothiocyanates (whose reactions are slower and yield products that fade relatively fast during irradiation) and fluorescamine (which reacts primarily with amine groups, but whose products also fade relatively rapidly).
Has been used to label E. coli thioredoxin. Weakly fluorescent but reacts with thiols to yield highly fluorescent stable thioethers.
Warning
Toxicity: Standard Handling (A)
Preparation Note
Recommended stock solution is 13 mg/ml (50 mM) in acetonitrile.
Reconstitution
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
Other Notes
Mansoor, M.A., et al. 1992. Anal. Biochem.200, 218.
Zangerle, L., et al. 1992. J. Neurochem.59, 181.
Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys.290, 1.
Martin, R.L., et al. 1989. J. Biol. Chem.264, 11768.
Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun.152, 1.
Chinn, P.C. 1985. Fed. Proc.44, 1620.
Zangerle, L., et al. 1992. J. Neurochem.59, 181.
Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys.290, 1.
Martin, R.L., et al. 1989. J. Biol. Chem.264, 11768.
Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun.152, 1.
Chinn, P.C. 1985. Fed. Proc.44, 1620.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
THIOLYTE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service