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B4380

Sigma-Aldrich

Bromobimane

≥97% purity, powder

Synonym(s):

Monobromobimane

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100 EA
$84.80

About This Item

Empirical Formula (Hill Notation):
C10H11BrN2O2
CAS Number:
Molecular Weight:
271.11
Beilstein/REAXYS Number:
4430959
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

$84.80


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Product Name

Bromobimane, ≥97% (HPLC)

Quality Level

assay

≥97% (HPLC)

form

powder

color

yellow

mp

161 °C

solubility

acetonitrile: 20 mg/mL

ε (extinction coefficient)

4.6-5.1 at 396-398 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

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1 of 4

This Item
1.250371.250361.25039
format

single component solution

format

single component solution

format

-

format

single component solution

agency

traceable to NIST

agency

traceable to NIST

agency

traceable to NIST

agency

traceable to NIST

specific analyte(s)

nitrate

specific analyte(s)

nitrate

specific analyte(s)

nitrate

specific analyte(s)

nitrate

concentration

15.0 mg/L (NO3-N in H2O)

concentration

2.50 mg/L (NO3-N in H2O)

concentration

0.50 mg/L (NO3-N in H2O)

concentration

40.0 mg/L (NO3-N in H2O)

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

Application

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.
Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro.[1] It has been used for the labeling of proteins containing thiol groups.[2][2]

Biochem/physiol Actions

Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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