71975-M
KOD Xtreme Hot Start DNA Polymerase
Ultra high fidelity dnapolymerase designed for the most challenging PCR applications including: crude sample PCR, amplification of long strand and highly GC-rich DNA templates.
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Quality Level
form
liquid
usage
sufficient for 200 reactions
feature
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included
hotstart
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
PCR: suitable
input
crude DNA
suitability
suitable for PCR
storage temp.
−20°C
Related Categories
General description
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
The KOD Xtreme<TMSYMBOL></TMSYMBOL> Hot Start DNA Polymerase* kit is an optimized PCR enzyme for the amplification of long or GC-rich DNA templates, including mouse tail-tips, plant tissues and synthetic biology applications. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen Perfectly Blunt<TMSYMBOL></TMSYMBOL> and LIC Vector Kits.
Features and Benefits
- Optimized for the highest PCR success rate, even with the most difficult targets.
- 10X higher fidelity than Taq blends
- Efficiently amplifies up to 90% GC-content DNA templates
- Amplifies genomic targets up to 24 kbp
- Amplifies phage/Lambda targets up to 40 kbp
- Eliminates mispriming and primer-dimer formation
- Convenient ambient-temperature setup compatible with automation
Components
- 1 × 200 U KOD Xtreme<TMSYMBOL></TMSYMBOL> Hot Start DNA Polymerase
- 3 × 1.7 mL 2X Xtreme Buffer
- 2 × 1 mL dNTPs (2 mM each)
Warning
Unit Definition
Legal Information
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Certificates of Analysis (COA)
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Articles
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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