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AB10911

Sigma-Aldrich

Anti-Osteocalcin Antibody

serum, from rabbit

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Synonym(s):
Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, bone gamma-carboxyglutamate (gla) protein (osteocalcin)
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BGLAP(632)
mouse ... Bglap(12096)
rat ... Bglap(25295)

General description

Osteocalcin belongs to the osteocalcin/matrix Gla protein family. It constitutes 1 to 2% of the total bone protein. It is made by osteoblasts, and is a major component of the noncollagenous bone matrix. It has a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. Drugs acting primarily on bone resorption, such as bisphosphonates, induce a rapid decline in specific bone resorption markers, whereas osteocalcin levels only change after extended therapy periods, in accordance with the expected dynamics of a bone formation marker. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget′s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

Specificity

The antibody reacts with the osteocalcin protein.

Immunogen

Epitope: aa 59-74
Synthetic peptide corresponding to aa 59-74 of osteocalcin protein.

Application

Detect Osteocalcin using this Anti-Osteocalcin Antibody validated for use in WB, IH.

Quality

Routinely evaluated by Western Blot on human heart lysate.

Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteocalcin in 10 μg of human heart lysate.

Target description

11 kDa calculated.
*Band of ~22 kDa is observed. The difference between observed and expected band may be due to post-translational modifications.

Linkage

Replaces: MABD182

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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