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AB1870

Sigma-Aldrich

Anti-Osteopontin Antibody

serum, Chemicon®

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Synonym(s):
Uropontin, Bone sialoprotein 1, SPP1/CALPHA1 fusion, Urinary stone protein, bone sialoprotein I, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialop
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SPP1(6696)

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This Item
O7264AB10910SAB4502841
Anti-Osteopontin Antibody serum, Chemicon®

AB1870

Anti-Osteopontin Antibody

Anti-Osteopontin Antibody serum, from rabbit

AB10910

Anti-Osteopontin Antibody

antibody form

serum

antibody form

affinity isolated antibody

antibody form

serum

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

Gene Information

human ... SPP1(6696)

Gene Information

human ... SPP1(6696)

Gene Information

human ... SPP1(6696)

Gene Information

human ... SPP1(6696)

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using human kidney, western blot: 0.5-1 μg/mL using whole extract of human embryonal kidney 293T cells and a chemiluminescent detection reagent

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

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General description

Osteopontin is a single-chain polypeptide with a molecular weight of approximately 35 kDa. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.

Specificity

Expected to cross-react with rat.
Human osteopontin. Does not cross react with osteonectin or human bone sialoprotein II.

Immunogen

Synthetic peptide corresponding to amino acids 75-90 of human osteopontin (SwissProt Accession # P10451; PSKSNESHDHMDDM DD).

Application

Immunohistochemistry:
A previous lot of this antibodu was used at a 1:1500 dilution on paraffin-embedded tissue sections.

ELISA:
A previous lot of this antibody was used at a 1:1000 dilution.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Osteobiology
This Anti-Osteopontin Antibody is validated for use in ELISA, IH, IH(P), WB for the detection of Osteopontin.

Quality

Routinely evaluated by Western Blot on RAW 264.7 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected osteopontin on 10 μg of RAW 264.7 lysates.

Target description

~35 kDa

Physical form

Rabbit polyclonal antiserum in buffer containing no preservatives.
Unpurified

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
POSITIVE CONTROL: Human urine (2-5 µg/mL normal concentration).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Nazwin Basheer et al.
European endodontic journal, 6(3), 295-302 (2021-12-31)
The aim of this study was to comparatively evaluate the proliferation, differentiation and mineralization inducing potential of strontium incorporated tetracalcium phosphate cement (STTCP) and mineral trioxide aggregate (MTA) on human dental pulp stem cells (hDPSCs). hDPSCs were cultured from freshly
Serum osteopontin predicts degree of hepatic fibrosis and serves as a biomarker in patients with hepatitis C virus infection.
Matsue, Y; Tsutsumi, M; Hayashi, N; Saito, T; Tsuchishima, M; Toshikuni, N; Arisawa, T; George, J
Testing null
Sequential application of steady and pulsatile medium perfusion enhanced the formation of engineered bone.
Correia, C; Bhumiratana, S; Sousa, RA; Reis, RL; Vunjak-Novakovic, G
Tissue Engineering: Part A null
Warren L Grayson et al.
Tissue engineering. Part A, 14(11), 1809-1820 (2008-07-16)
We describe a novel bioreactor system for tissue engineering of bone that enables cultivation of up to six tissue constructs simultaneously, with direct perfusion and imaging capability. The bioreactor was used to investigate the relative effects of initial seeding density
Mirjam Fröhlich et al.
Tissue engineering. Part A, 16(1), 179-189 (2009-08-15)
We report engineering of half-centimeter-sized bone constructs created in vitro using human adipose-derived stem cells (hASCs), decellularized bone scaffolds, and perfusion bioreactors. The hASCs are easily accessible, can be used in an autologous fashion, are rapidly expanded in culture, and

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