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AB1529

Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

serum, Chemicon®

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Synonym(s):
NOS I, bNOS, nNOS
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rabbit, rat, guinea pig

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

guinea pig ... Nos1(100729682)
rabbit ... Nos1(100009243)
rat ... Nos1(24598)

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This Item
N218AB1632AB16301
Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

biological source

sheep

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

guinea pig ... Nos1(100729682)
rabbit ... Nos1(100009243)
rat ... Nos1(24598)

Gene Information

mouse ... Nos1(18125)
rat ... Nos1(24598)

Gene Information

mouse ... Nos1(18125)

Gene Information

human ... NOS3(4846)

UniProt accession no.

P29475

UniProt accession no.

P29476

UniProt accession no.

P29475

UniProt accession no.

P29474

clone

polyclonal

clone

NOS-3F7-B11-B5, monoclonal

clone

polyclonal

clone

polyclonal

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Specificity

bNOS (neuronal). Not yet tested for cross-reactivity to iNOS or eNOS. See reference below.

Immunogen

A 21 amino acid peptide (1409-1429) of the neuronal form of NOS from rat cerebellum, coupled to KLH. The sequence is H-RSESIAFIEESKKDADEVFSS-NH2.

Application

Detect Nitric Oxide Synthase I using this Anti-Nitric Oxide Synthase I Antibody validated for use in WB, IH.
Immunohistochemistry: 1:500-1,000

Immunoblotting

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Siomara Hernandez et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(5), 6168-6184 (2019-02-23)
Gulf War illness (GWI) is a chronic multisymptom disorder that is prominent in Gulf War veterans. Major unexplained symptoms of GWI include functional gastrointestinal disorders and undiagnosed illnesses, including neurologic disorders. Exposure to the antinerve gas drug pyridostigmine bromide (PB)
Isola A M Brown et al.
American journal of physiology. Gastrointestinal and liver physiology, 314(1), G39-G52 (2017-09-09)
Enteric glia play an important neuroprotective role in the enteric nervous system (ENS) by producing neuroprotective compounds such as the antioxidant reduced glutathione (GSH). The specific cellular pathways that regulate glial production of GSH and how these pathways are altered
Eileen S Rodriguez-Tapia et al.
Experimental physiology, 102(3), 299-313 (2016-12-23)
What is the central question of this study? Subtypes of enteric neurons are coded by the neurotransmitters they synthesize, but it is not known whether enteric neuron subtypes might also be coded by other proteins, including calcium channel subtypes controlling
Viviana Filpa et al.
American journal of physiology. Gastrointestinal and liver physiology, 312(4), G374-G389 (2017-02-06)
Neuronal and inducible nitric oxide synthase (nNOS and iNOS) play a protective and damaging role, respectively, on the intestinal neuromuscular function after ischemia-reperfusion (I/R) injury. To uncover the molecular pathways underlying this dichotomy we investigated their possible correlation with the
Michela Bistoletti et al.
PeerJ, 8, e8442-e8442 (2020-02-26)
Inflammatory bowel diseases are associated with remodeling of neuronal circuitries within the enteric nervous system, occurring also at sites distant from the acute site of inflammation and underlying disturbed intestinal functions. Homeoproteins orthodenticle OTX1 and OTX2 are neuronal transcription factors

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