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Key Documents

AB1554

Sigma-Aldrich

Anti-Nerve Growth Factor Receptor Antibody, p75

serum, Chemicon®

Synonym(s):

CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NGFR(4804)

General description

Nerve Growth Factor Receptor p75 (UniProt: Q9Z0W1; also known as Tumor necrosis factor receptor superfamily member 16, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, CD271) is encoded by the Ngfr (also known as Tnfrsf16) gene in murine species. Nerve Growth Factor Receptor p75 is a single-pass type I, homodimeric membrane protein that can serve as a low affinity receptor for NGF, BDNF, NT-3, and NT-4. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 22-246), a transmembrane domain (aa 247-265), and a cytoplasmic domain (aa 266-417). Its expression is shown to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and in liver and expression is seen at higher levels during the light period and lower during dark period. Depending on the developmental stage, cell type, and whether they are unbound or ligand bound, and the type of ligand, p75 can activate apoptosis or survival. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 346-411, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type.

Specificity

It has been reported that this antibody does not recognize rat p75 by Western blot. See MAB365 for rat reactive antibody using Western blotting.
p75 nerve growth factor receptor (p75 Neurotrophin receptor, low affinity neurotrophin receptor), extracellular domain.

Immunogen

Epitope: extracellular
Recombinant mouse NGF Receptor p75 extracellular fragment.

Application

Anti-Nerve Growth Factor Receptor Antibody, p75 is an antibody against Nerve Growth Factor Receptor for use in IC, IH(P),WB and IP.
Immunoprecipitation:
1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.

Immunohistochemistry(paraffin):
1:50 and 1:300 dilution from a previous lot detected NGF Receptor p75 using IHC-Select Detection with HRP-DAB.

Immunocytochemistry:
1:500 dilution from a previous lot detected NGF Receptor p75 in rat PC12 cells.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Quality

Routinely evaluated by immunoprecipitation on PC12 cell lysates.

Immunoprecipitation (IP): 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.

Target description

44.7 kDa

Linkage

Replaces: 04-1111

Physical form

Rabbit polyclonal serum containing 0.05% NaN3.
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat PC12 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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NMDA-dependent switch of proBDNF actions on developing GABAergic synapses.
Langlois, A; Diabira, D; Ferrand, N; Porcher, C; Gaiarsa, JL
Cerebral Cortex (1991)
Alexander Schulz et al.
Acta neuropathologica, 132(2), 289-307 (2016-05-30)
Schwannomas are predominantly benign nerve sheath neoplasms caused by Nf2 gene inactivation. Presently, treatment options are mainly limited to surgical tumor resection due to the lack of effective pharmacological drugs. Although the mechanistic understanding of Nf2 gene function has advanced
Victor Vendrell et al.
Development (Cambridge, England), 142(16), 2792-2800 (2015-07-15)
Transcriptional regulatory networks are essential during the formation and differentiation of organs. The transcription factor N-myc is required for proper morphogenesis of the cochlea and to control correct patterning of the organ of Corti. We show here that the Otx2
Michael D Kawaja et al.
The Journal of comparative neurology, 519(13), 2522-2545 (2011-04-02)
Nerve growth factor (NGF) and its precursor proNGF are perhaps the best described growth factors of the mammalian nervous system. There remains, however, a paucity of information regarding the precise cellular sites of proNGF/NGF synthesis. Here we report the generation
Minna M Wieck et al.
Tissue engineering. Part A, 22(1-2), 53-64 (2015-09-29)
Tissue-engineered colon (TEC) might potentially replace absent or injured large intestine, but the enteric nervous system (ENS), a key component, has not been investigated. In various enteric neuropathic diseases in which the TEC is derived from aganglionic donor colon, the

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