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MAB365

Sigma-Aldrich

Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG

clone 192-IgG, Chemicon®, from mouse

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Synonym(s):
CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

192-IgG, monoclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NGFR(4804)

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This Item
MAB390AB1554MAB5386X
antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

serum

antibody form

purified immunoglobulin

clone

192-IgG, monoclonal

clone

192, monoclonal

clone

polyclonal

clone

ME20.4, monoclonal

species reactivity

rat

species reactivity

rat

species reactivity

mouse, rat, human

species reactivity

rabbit, human, pig, sheep

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable

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General description

The low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.

Specificity

Reacts with rat p75 protein.
Specific for the rat low affinity Nerve Growth Factor-Receptor (p75), does not react with human or mouse p75.

Immunogen

Epitope: Extracellular
n-octoglucoside stabilized proteins containing p75 receptor, which were isolated from plasma membranes of PC-12 cells (Chandler et al., 1984).

Application

Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Western Blot:
1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.

Inhitibition Assay:
Inhibition on NGF binding {Chandler, 1984}.

Immunoprecipitation:
5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.

Immunohistochemistry:
1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.

Quality

Immunohistochemistry:
1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.

Target description

75 kDa

Linkage

Replaces: 04-1111

Physical form

Format: Purified
Mouse monoclonal IgG1 in 0.02M Phosphate buffer, pH7.6, 0.25M NaCl with 0.1% sodium azide
Protein A purified

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Western Blot: NGF-differentiated PC12 cell lysate.
Immunohistochemistry: Rat brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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Marta Volosin et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(39), 9870-9879 (2008-09-26)
Seizure-induced damage elicits a loss of hippocampal neurons mediated to a great extent by the p75 neurotrophin receptor (NTR). Proneurotrophins, which are potent apoptosis-inducing ligands for p75(NTR), were increased in the hippocampus, particularly in astrocytes, by pilocarpine-induced seizures; and infusion
Ying Li et al.
Cell transplantation, 25(2), 293-299 (2015-07-02)
Cell transplantation is one of the most promising strategies for repair of human spinal cord injuries. Animal studies from a number of laboratories have shown that transplantation of olfactory ensheathing cells cultured from biopsies of the olfactory bulb mediate axonal
A Petersen et al.
Nature communications, 9(1), 4430-4430 (2018-10-27)
Biomaterials developed to treat bone defects have classically focused on bone healing via direct, intramembranous ossification. In contrast, most bones in our body develop from a cartilage template via a second pathway called endochondral ossification. The unsolved clinical challenge to
Mary Ann Asson-Batres et al.
The Journal of comparative neurology, 496(2), 149-171 (2006-03-16)
Work from our laboratory suggests that retinoic acid (RA) influences neuron development in the postnatal olfactory epithelium (OE). The studies reported here were carried out to identify and localize retinaldehyde dehydrogenase (RALDH) expression in postnatal rat OE to gain a
Tara Lovekamp-Swan et al.
Brain research, 1159, 54-66 (2007-06-22)
Estrogen is a powerful neuroprotective agent with the ability to induce trophic and antiapoptotic genes. However, concerns about negative overall health consequences of estrogen replacement after menopause have led to the adoption of other strategies to obtain estrogen's benefits in

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