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N8773

Sigma-Aldrich

Anti-Nerve Growth Factor-β antibody produced in goat

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-NGF-β
MDL number:
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

rat

technique(s)

capture ELISA: 0.2-0.8 μg/mL
immunohistochemistry: 5-15 μg/mL
neutralization: 0.02-0.1 μg/mL (in the presence of 10.0μg/ml of recombinat rat β-NGF using the human erythroleukemic TF-1 cell line.)
western blot: 0.1-0.2 μg/mL

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Ngf(310738)

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Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

fractionated antiserum

antibody form

affinity isolated antibody

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

Gene Information

rat ... Ngf(310738)

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

Gene Information

rat ... Ngfr(24596)

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General description

Nerve Growth factor (NGF) is a growth factor that is essential for the development and maintenance of the nervous system. NGF is composed of three non-covalently linked α, β and γ subunits. The β subunit is responsible for the biological functions of NGF. Nerve growth factor (NGF) binds to either to NGF receptor (NGFR) or the Trk receptor and can elicit biological responses through each of the receptors independently. NGF is important in the survival and differentiation of neurons and neural crest-derived cells during embryonic and early postnatal development and maintenance of peripheral neurons. NGF is also implicated in inflammation and allergic reactions . Anti-Nerve Growth Factor-β antibody recognizes rat NGF-β. The antibody shows less than 1% cross reactivity with recombinant human β-NGF.

Immunogen

recombinant rat β-nerve growth factor expressed in Sf 21 cells.

Application

Anti-NGF-β antibody may be used for neutralization assays; the ND50 is ~0.02-0.1 μg/ml. For immunoblotting the recommended antibody concentration is 0.1-0.2 μg/ml. The antibody is suitable for immunohistochemistry at a working concentration of 5-15 μg/ml. For ELISA, antibody concentration of 0.2-0.8 μg/ml may be used. Antibody dilution of 1:500 was used for immunoblotting of extracts of rat nerve cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Bopaiah P Cheppudira et al.
BMC anesthesiology, 16(1), 73-73 (2016-09-07)
Nerve growth factor (NGF) is known to induce inflammation and pain; however its role in opioid-induced tolerance has not been studied. This study investigated the effects of an anti-NGF neutralizing antibody on the development of tolerance following chronic morphine treatment
Margherita Maranesi et al.
Biology of reproduction, 98(5), 634-643 (2018-02-14)
To investigate the ovulatory mechanisms triggered by raw semen (RS) in rabbits, we examined the expression of nerve growth factor (NGF)-a supposed ovulation-inducing factor (OIF)-and cognate receptors in anterior pituitary, ovary, and cervix as well as plasma NGF and luteinizing
Ana Sanchez-Rodriguez et al.
PloS one, 14(7), e0219780-e0219780 (2019-07-19)
In some induced-ovulating species, beta nerve growth factor (β-NGF) has important roles in ovulation, though data for rabbits are still inconclusive. In this study we first synthesized functional recombinant β-NGF from rabbit tissue (rrβ-NGF) to address the following objectives: 1)
Ana Sanchez-Rodriguez et al.
Theriogenology, 126, 206-213 (2018-12-24)
β-Nerve Growth Factor (β-NGF) is a neurotrophin which acts through its receptors TrkA and p75, performing important actions in male reproductive physiology and its presence in seminal plasma (SP) has been related to male fertility. The aim of the present
Uros Kovacic et al.
Neurobiology of aging, 31(2), 339-350 (2008-05-24)
Sprouting of uninjured nociceptive axons was examined in young adult, middle aged and aged rats. Axon sprouting from the spared sural nerve, both into adjacent denervated skin and into end-to-side coapted nerve graft, was significantly higher in young rats than

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