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AB1954

Sigma-Aldrich

Anti-Fibronectin Antibody

Chemicon®, from rabbit

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Synonym(s):
Cold-insoluble globulin, fibronectin 1, migration stimulating factor, migration-stimulating factor
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

Related Categories

General description

Fibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.

Specificity

Other species have not been tested.
The antibody reacts with the 262 kDa fibronectin protein.

Immunogen

Purified rat plasma fibronectin.

Application

Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Quality

Routinely evaluated by Western Blot on normal rat serum.

Western Blot Analysis:
0.05 μg/mL - 1 μg/mL antibody detected fibronectin in 15 μL of normal rat serum.

Target description

262 kDa

Physical form

Antigen Affinity Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot: Normal rat serum

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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