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AB5032

Sigma-Aldrich

Anti-Neural Cell Adhesion Molecule Antibody

Chemicon®, from rabbit

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Synonym(s):
CD56 antigen, antigen recognized by monoclonal antibody 5.1H11
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, chicken, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NCAM1(4684)

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This Item
WH0004684M1MAB2120ZC9672
biological source

rabbit

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable using cells and tissues, western blot: 1:100 using a freshly prepared extract from newborn and adult rat brain, or rat cerebral cortex extract

species reactivity

mouse, rat, chicken, human

species reactivity

human

species reactivity

human, pig

species reactivity

rat, human

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

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General description

The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
The previously assigned protein identifier P13592 has been merged into P13591. Full details can be found on the UniProt database.

Specificity

Expected to cross-react with Chicken, Human, and rat.
Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.

Immunogen

Highly purified chicken NCAM

application

Detect Neural Cell Adhesion Molecule using this Anti-Neural Cell Adhesion Molecule Antibody validated for use in ELISA, IF, IH, FUNC & WB.
Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.

Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.

Function blocking:
A previous lot of this antibody was used in Function blocking.

ELISA:
0.3 μg/mL of a previous lot was used.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.

Target description

MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa A breakdown product of 160 kDa may also be visible.

Physical form

ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS containing no preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Neuraminidase-treated mouse brain membrane.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Taste bud cells of adult mice are responsive to Wnt/?-catenin signaling: implications for the renewal of mature taste cells.
Gaillard, D; Barlow, LA
Genesis (2000)
Protein kinase A activation promotes plasma membrane insertion of DCC from an intracellular pool: A novel mechanism regulating commissural axon extension.
Bouchard, JF; Moore, SW; Tritsch, NX; Roux, PP; Shekarabi, M; Barker, PA; Kennedy, TE
The Journal of Neuroscience null
Francisco Barrionuevo et al.
Developmental biology, 327(2), 301-312 (2009-01-07)
Sox9 and Sox8 are transcription factors expressed in embryonic and postnatal Sertoli cells of the mouse testis. Sox9 inactivation prior to the sex determination stage leads to complete XY sex reversal. In contrast, there is normal embryonic testis development in
Maria S Skog et al.
Stem cell research & therapy, 7(1), 113-113 (2016-08-17)
In order to develop novel clinical applications and to gain insights into possible therapeutic mechanisms, detailed molecular characterization of human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) is needed. Neural cell adhesion molecule (NCAM, CD56) is a transmembrane glycoprotein modulating cell-cell
Xuying Zhang et al.
Frontiers in neural circuits, 11, 19-19 (2017-04-08)
The neural cell adhesion molecule (NCAM), has been shown to be an obligate regulator of synaptic stability and pruning during critical periods of cortical maturation. However, the functional consequences of NCAM deletion on the organization of inhibitory circuits in cortex

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