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AB5220

Sigma-Aldrich

Anti-Growth Associated Protein-43 (GAP-43) Antibody

Chemicon®, from rabbit

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Synonym(s):
Neuromodulin
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse (high degree), rat, bovine, chicken, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GAP43(2596)

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AB5312AB5401MAB347
antibody form

purified antibody

antibody form

serum

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

9-1E12, monoclonal

species reactivity

mouse (high degree), rat, bovine, chicken, human

species reactivity

mouse, human, monkey, rat, feline, bovine

species reactivity

rat

species reactivity

feline, rat, hamster

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

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General description

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Specificity

Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
Recognizes Growth Associated Protein-43 (GAP-43).

Immunogen

Recombinant rat GAP-43 (complete sequence).

Application

Anti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.
Immunohistochemistry:
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.

Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.

Immunoprecipitation:
1:100 dilution of a previous lot was used.

ELISA:
1:2,000 dilution of a previous lot was used.

Quality

Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.

Target description

23.6 kDa

Physical form

Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.

Storage and Stability

Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Analysis Note

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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