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Key Documents

AB825

Sigma-Aldrich

Anti-Apolipoprotein J Antibody

Chemicon®, from goat

Synonym(s):

Clusterin, ApoJ

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CLU(1191)

Specificity

Apolipoprotein J, Human. Negligible cross reactivity to Apo A-I, A-II, B, C-I, C-II, C-III, and E by ELISA. Negligible non-specific cross-reaction with other human serum proteins.

Immunogen

Apolipoprotein J, purified from human plasma.

Application

Anti-Apolipoprotein J Antibody is an antibody against Apolipoprotein J for use in ELISA, IP, WB, IH(P).
Immunoblotting: 1:5,000-1:10,000

Immunohistochemistry on paraffin embedded tissue: 1:50-1:200

Immunoprecipitation

ELISA: 1:5,000-1:10,000

Optimal working dilutions must be determined by end user.
Research Category
Metabolism
Research Sub Category
Lipid Metabolism & Weight Regulation

Physical form

Affinity purified immunoglobulin. Liquid in 0.125 M Sodium Borate buffer, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, with 0.01% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for several weeks. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for up to 12 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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N Bahia El Idrissi et al.
Clinical and experimental immunology, 184(3), 338-346 (2016-01-11)
Mycobacterium leprae infection gives rise to the immunologically and histopathologically classified spectrum of leprosy. At present, several tools for the stratification of patients are based on acquired immunity markers. However, the role of innate immunity, particularly the complement system, is
Svetlana Hakobyan et al.
Journal of Alzheimer's disease : JAD, 54(2), 707-716 (2016-08-29)
There is a critical unmet need for reliable markers of disease and disease course in mild cognitive impairment (MCI) and early Alzheimer's disease (AD). The growing appreciation of the importance of inflammation in early AD has focused attention on inflammatory
Samantha L Hersrud et al.
The FEBS journal, 283(3), 459-471 (2015-11-14)
The neuronal ceroid lipofuscinoses (NCLs) are a group of neurodegenerative genetic diseases that primarily affect children and have no known cure. A unified clinical rating scale for the juvenile form of NCL has been developed, although it has not been
Chera L Maarouf et al.
PloS one, 6(11), e27291-e27291 (2011-11-17)
The amyloid cascade hypothesis provides an economical mechanistic explanation for Alzheimer's disease (AD) dementia and correlated neuropathology. However, some nonagenarian individuals (high pathology controls, HPC) remain cognitively intact while enduring high amyloid plaque loads for decades. If amyloid accumulation is
Chera L Maarouf et al.
PloS one, 9(8), e105784-e105784 (2014-08-29)
Defining the biochemical alterations that occur in the brain during "normal" aging is an important part of understanding the pathophysiology of neurodegenerative diseases and of distinguishing pathological conditions from aging-associated changes. Three groups were selected based on age and on

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