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ABC142

Sigma-Aldrich

Anti-Pex14 (peroxisomal membrane marker) Antibody

serum, from rabbit

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Synonym(s):
Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PEX14(5195)

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This Item
SAB4502176ABC143WH0005193M1
antibody form

serum

antibody form

affinity isolated antibody

antibody form

serum

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

2G6, monoclonal

species reactivity

mouse, rat, human

species reactivity

rat, human, mouse

species reactivity

mouse, rat, human

species reactivity

human

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

western blot: suitable

technique(s)

indirect ELISA: suitable

NCBI accession no.

NP_004556.1

NCBI accession no.

O75381

NCBI accession no.

NP_002609.1

NCBI accession no.

-

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General description

Peroxisomal membrane protein 14 (PEX14) is a component of the peroxisomal translocation machinery. It interacts with PEX19 and PEX13, and functions as the peroxisomal membrane docking factor for PEX5. The PEX7-PTS2 complex also docks at PEX14 indirectly via PEX5L, the longer isoform of PEX5. Defects in PEX14 result in peroxisome biogenesis disorder complementation group K and Zellweger syndrome.

Immunogen

His-tagged recombinant protein corresponding to human PEX14.

Application

Detect Pex14 (peroxisomal membrane marker) using this Anti-Pex14 (peroxisomal membrane marker) Antibody validated for use in Western Blotting, ICC.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PEX14 (peroxisomal membrane marker) in 10 µg of human liver tissue lysate.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected PEX14 (peroxisomal membrane marker) in Pex14p-transfected CHO cells (Pinto, M. P., et al. 2006. J Biol Chem. 281(45):34492-34502.).

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: A 1:500 dilution from a representative lot detected PEX14 (peroxisomal membrane marker) in 10 µg of NIH/3T3 cell lysate.

Target description

~60 kDa observed. This product is known to exist in two isoforms at 41 kDa and 37 kDa, but it has also been observed at ~57-60 kDa (Nguyen, T. et al. 2006. J Cell Sci. 119 (Pt 4):636-645). An uncharacterized band may be observed at ~51 kDa in some cell lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
NIH/3T3 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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