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ABD116

Sigma-Aldrich

Anti-Human Oct-4 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
POU domain,class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3
eCl@ss:
32160702
NACRES:
NA.75

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), goat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)

General description

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

Specificity

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

Immunogen

Epitope: unknown
KLH-EDC-conjugated linear peptide corresponding to the unknown of Human Oct-4.

Application

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected human Oct-4 in 10 µg of H9 human ES cells.
Research Category
Stem Cell Research

Stem Cell Research
Research Sub Category
Neural Stem Cells

Developmental Signaling
This Anti-Human Oct-4 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Human Oct-4.

Quality

Evaluated by Western Blotting in H9 human ES cell lysate and mouse ES cells lysate.

Western Blotting Analysis: A 0.25 µg/mL dilution of this antibody detected human Oct-4 in 10 µg of H9 human ES cell lysate but not in 10 µg of mouse ES cells lysate.

Target description

~40 kDa observed

Physical form

Antigen Affinity Purified
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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