ABE290
Anti-TET3
from rabbit, purified by affinity chromatography
Synonym(s):
Methylcytosine dioxygenase TET3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology), rat (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology)
technique(s)
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... TET3(200424)
General description
MABE1144/1132/1133 Methylcytosine dioxygenase TET3 (EC 1.14.11.n2; UniProt O43151) is encoded by the TET3 (also known as KIAA0401) gene (Gene ID 200424) in human. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported. Three TET3 spliced isoforms/variants have been reported, the long or full-length isoform Tet3FL and the short isoform Tet3s are upregulated during embryonic stem cell (ESC) differentiation toward the neuronal lineage, while Tet3o is oocyte-specific and not detected in ESCs or in any other cell types or adult mouse tissues tested, indicating a role of Tet3o in paternal genome oxidation in mouse zygotes. Tet3FL and Tet3o contain the entire Tet3s sequence with additional (but different) N-terminal sequence. Only Tet3FL contains an N-terminal CXXC domain that binds to unmethylated CpGs and exhibits highest affinity toward 5caC. Tet3FL is substantially less active than Tet3o and Tet3s, suggesting that the CXXC domain restricts Tet3FL 5mC oxidation capacity.
Specificity
Immunogen sequence is present in TET3 isoforms Tet3FL, Tet3s, and Tet3o spliced isoforms reported by Jin, S.G., et al. (2016). Cell Rep. 14(3):493-505), as well as all additional human and mouse TET3 spliced isoforms reported by UniProt (O43151, Q8BG87). Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
Immunogen
KLH-conjugated linear peptide corrsponding to an internal sequence within the N-terminal half of human TET3.
Application
Anti-TET3, Cat. No. ABE290, is a highly specific rabbit polyclonal antibody that targets methylcytosine dioxygenase TET3 and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated the exogenously expressed mouse TET3-GFP fusion protein from 500 µg lysate of transfected mouse embryonic stem cells (mESCs).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected TET3 chromosome enrichment sites by ChIP-seq analysis (10 µg per ChIP) using chromatin preparation from mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 oocyte isoform Tet3o (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected the immunoreactivity of TET3 oocyte isoform Tet3o in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse zygotes (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunoprecipitation Analysis: 10 µg from a representative lot co-immunoprecipitated endogenous OGT with the exogenously overexpressed mouse TET3 from transfected mouse embryonic stem cells (mESCs) by RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected TET3 chromosome enrichment sites by ChIP-seq analysis (10 µg per ChIP) using chromatin preparation from mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse embryonic stem cells (mESCs) transfected to overexpress mouse TET3 oocyte isoform Tet3o (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected the immunoreactivity of TET3 oocyte isoform Tet3o in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized mouse zygotes (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Immunoprecipitation Analysis: 10 µg from a representative lot co-immunoprecipitated endogenous OGT with the exogenously overexpressed mouse TET3 from transfected mouse embryonic stem cells (mESCs) by RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) (Courtesy of Reik lab, Epigenetics ISP, Babraham Institute, UK).
Quality
Evaluated by Western Blotting of mouse TET3 GFP fusion.
Western Blotting Analysis: 2 µg/mL of this antibody detected the exogenously expressed mouse TET3 GFP fusion protein in 10 µg of lysate from transfected mouse embryonic stem cells (mESCs).
Western Blotting Analysis: 2 µg/mL of this antibody detected the exogenously expressed mouse TET3 GFP fusion protein in 10 µg of lysate from transfected mouse embryonic stem cells (mESCs).
Target description
193.7/179.4 kDa (human isoform Tet3FL/Tet3s), 194.8/180.4/185.1 kDa (mouse isoform Tet3FL/Tet3s/Tet3o), 166.7/77.47 kDa (UniProt human isoform 2/3), 76.26 kDa (UniProt mouse isoform 2) calculated. Note that UniProt isoform 1 (O43151-1/human and Q8BG87-1/mouse) corresponds to isoform Tet3s, not Tet3FL (NM_001287491.1/human and JX946278.1/mouse).
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt..
Other Notes
Concentration: Please refer to lot specific datasheet.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Juan Li et al.
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Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1) is a deubiquitinating enzyme that regulates ERα expression in triple-negative cancer (TNBC). This study aimed to explore the deubiquitination substrates of UCHL1 related to endocrine therapeutic responses and the mechanisms of UCHL1 dysregulation in TNBC.
Harmony C Ketchum et al.
Communications biology, 7(1), 415-415 (2024-04-06)
The ten-eleven-translocation family of proteins (TET1/2/3) are epigenetic regulators of gene expression. They regulate genes by promoting DNA demethylation (i.e., catalytic activity) and by partnering with regulatory proteins (i.e., non-catalytic functions). Unlike Tet1 and Tet2, Tet3 is not expressed in
A small-molecule degrader of TET3 as treatment for anorexia nervosa in an animal model.
Lv, et al.
Proceedings of the National Academy of Sciences of the USA, 120, e2300015120-e2300015120 (2023)
Aixia Liu et al.
iScience, 24(9), 103065-103065 (2021-09-28)
A conceptual framework for understanding abnormal endometrial decidualization, with considerable significance for the diagnosis and treatment of abnormal decidualization-related changes in non-receptive endometrium in implantation failure during early pregnancy is very important. Here, we found the expression levels of miR-29a
Yetao Xu et al.
Cell reports, 30(5), 1310-1318 (2020-02-06)
Pathological activation of TGF-β signaling is universal in fibrosis. Aberrant TGF-β signaling in conjunction with transdifferentiation of hepatic stellate cells (HSCs) into fibrogenic myofibroblasts plays a central role in liver fibrosis. Here we report that the DNA demethylase TET3 is
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