Skip to Content
MilliporeSigma
All Photos(2)

Documents

ABE347

Sigma-Aldrich

Anti-NFκB p65 Antibody, CT

from rabbit

Synonym(s):

v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), Nuclear factor of kappa light polypeptide gene enhancer

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μg

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... RELA(5970)

General description

The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.

Specificity

This antibody recognizes NFκB at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human NFκB.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-NFκB p65 Antibody, CT (Rabbit Polyclonal Antibody) validated in WB, IP to detect NFκB p65 also known as transcription factor p65.
Western Blot (SNAP i.d.) Analysis: 5 µg/mL from a representative lot detected NFκB on 10 µg of A431 cell lysate.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated NFκB from A431 RIPA lysate.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NFκB in 10 µg of A431 cell lysate.

Target description

~65 kDa observed.
Four isoforms produced by alternative splicing have been described: Isoform 1 (p65) at 60.2 kDa, Isoform 2 (p65 delta 2) at 58.8 kDa, Isoform 3 (p65 delta) at 59.1 kDa, and Isoform 4 at 59.9 kDa

Linkage

Replaces: 06-418

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sandra Gegunde et al.
ACS chemical neuroscience, 12(13), 2336-2346 (2021-06-11)
Anhydroexfoliamycin, a secondary metabolite from Streptomyces, has shown antioxidant properties in primary cortical neurons reducing neurodegenerative hallmarks diseases, both in vitro and in vivo models. Activated microglia, in the central nervous system, plays a crucial role in neuroinflammation and is
Jingjing Lu et al.
Frontiers in pharmacology, 13, 838873-838873 (2022-04-05)
Tetrastigma hemsleyanum Diels et Gilg (Sanyeqing, SYQ) has traditionally been used to treat inflammation, high fever and improve immune function of patients. Polysaccharides have been proved to be one of the important components of SYQ. Previous studies have confirmed the
Xin Shen et al.
International heart journal, 63(5), 881-892 (2022-10-03)
Ischemic heart disease (IHD) is a prominent global cause of morbidity and death resulting from the narrowing or blockage of cardiac coronary arteries. Exposing isolated cardiac myocytes to hypoxia-reoxygenation (H/R) might be an efficient tool to investigate the etiology and
Pomegranate juice exacerbates oxidative stress and nigrostriatal degeneration in Parkinson's disease.
Tapias, V; Cannon, JR; Greenamyre, JT
Neurobiology of Aging null
Shekhar Saha et al.
Cancer research, 80(5), 950-963 (2020-01-05)
DRAIC is a 1.7 kb spliced long noncoding RNA downregulated in castration-resistant advanced prostate cancer. Decreased DRAIC expression predicts poor patient outcome in prostate and seven other cancers, while increased DRAIC represses growth of xenografted tumors. Here, we show that

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service