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Monoclonal Anti-NF-κB antibody produced in mouse

clone NF-12, ascites fluid

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Anti-CVID12, Anti-EBP-1, Anti-KBF1, Anti-NF-kB, Anti-NF-kB1, Anti-NF-kappa-B1, Anti-NF-kappaB, Anti-NF-kappabeta, Anti-NFKB-p105, Anti-NFKB-p50, Anti-NFkappaB
MDL number:

biological source


Quality Level



antibody form

ascites fluid

antibody product type

primary antibodies


NF-12, monoclonal

mol wt

antigen 65 kDa


15 mM sodium azide

species reactivity

human, mouse


immunocytochemistry: suitable
microarray: suitable
western blot: 1:1,000 using a whole cell extract from a mouse fibroblast cell line



UniProt accession no.

shipped in

dry ice

storage temp.


target post-translational modification


Gene Information

human ... NFKB1(4790)
mouse ... Nfkb1(18033)

General description

Monoclonal Anti-NF-κB p65 (mouse IgG1 isotype) is derived from the NF-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant, mouse NF-κB p65 fragment. Nuclear factor κB (NF-κB1) is a member of NF-κB/Rel family of transcription factors.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) is a subunit of nuclear factor κB (NF-κB) and exists as a dimer. It is expressed in the cytosol.


recombinant mouse NF-κB p65 fragment (C-terminal, 151 amino acids).


Monoclonal Anti-NF-κB antibody produced in mouse has been used in immunohistochemistry and Western blotting.

Biochem/physiol Actions

Nuclear factor kappa-B (NF-κB1) has an ability to affect transcription of κB reporter genes positively or negatively when assayed following transfection.
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 (NFKB1) has a role in hippocampal neurogenesis. It regulates the signaling by nuclear factor κB (NF-κB). Deletion of the gene in mice leads to defects in innate and adaptive immunity. The protein acts as a mediator of apoptotic response to S-phase DNA damage and replication stress. NFKB1 modulates the structural plasticity of cortical neurons. Knockout of the protein in mice has been shown to cause liver apoptosis and embryonic death.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids




Not applicable


Not applicable

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