Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

ABE457

Sigma-Aldrich

Anti-c-Fos Antibody

rabbit polyclonal

Synonym(s):

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

product name

Anti-c-Fos Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

sheep (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_005243

UniProt accession no.

P01100

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FOS(2353)

General description

c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Specificity

This antibody recognizes the N-terminus of c-Fos.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.

Application

Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.

Quality

Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.

Target description

~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)

Analysis Note

Control
PMA(TPA) treated HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Not finding the right product?  

Try our Product Selector Tool.

Comparable product

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Min-Yu Sun et al.
Scientific reports, 9(1), 16431-16431 (2019-11-13)
Pentameric GABAA receptors mediate a large share of CNS inhibition. The γ2 subunit is a typical constituent. At least 11 mutations in the γ2 subunit cause human epilepsies, making the role of γ2-containing receptors in brain function of keen basic
Anett J Nagy et al.
PLoS biology, 16(6), e2004712-e2004712 (2018-06-26)
Evolutionary development of vision has provided us with the capacity to detect moving objects. Concordant shifts of visual features suggest movements of the observer, whereas discordant changes are more likely to be indicating independently moving objects, such as predators or
Ozra Dehkordi et al.
Journal of alcoholism and drug dependence, 5(4) (2017-10-19)
Orexin/hypocretin-containing neurons in lateral hypothalamus (LH) are implicated in the neurobiology of nicotine addiction. However, the neuroanatomical relationships between orexin-neurons/nerve fibers and nicotine-activated cells within the reward-addiction neurocircuitry is not known. In the present study in mice, we first used
Isabel F Augur et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(39), 10174-10180 (2016-09-30)
The ventromedial prefrontal cortex (vmPFC) has been shown to negatively regulate cocaine-seeking behavior, but the precise conditions by which vmPFC activity can be exploited to reduce cocaine relapse are currently unknown. We used viral-mediated gene transfer of designer receptors (DREADDs)
Sydney Aten et al.
Neuropharmacology, 144, 256-270 (2018-10-21)
miR-132 and miR-212 are structurally-related microRNAs that are expressed from the same non-coding transcript. Accumulating evidence has shown that the dysregulation of these microRNAs contributes to aberrant neuronal plasticity and gene expression in the mammalian brain. Consistent with this, altered
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service