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MABE329

Sigma-Aldrich

Anti-c-Fos Antibody, clone 2G9C3

clone 2G9C3, from mouse

Synonym(s):

Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

2G9C3, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... FOS(2353)

General description

c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Specificity

This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins.

Immunogen

Linear peptide corresponding to human c-Fos.

Application

Anti-c-Fos Antibody, clone 2G9C3 is a Mouse Monoclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB, ICC.
Immunocytochemistry Analysis: A representative lot detected c-Fos in schwann cells. (Lafarga, M., et al. (2002). Molecular Biology of the Cell. 13:2771–2782.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos on 10 µg of HeLa cell lysate.

Target description

~55/62 kDa observed. This antibody recognizes the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. The calculated molecular wieght is 46 kDa; however, c-Fos has been observed at ~55 kDa in western blots. (Assim, M., et al. (2010). Journal of Biological Chemistry. 285(26):20343–20357.)

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ from cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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