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F7799

Sigma-Aldrich

Anti-c-Fos antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
C-Fos Antibody, C-Fos Antibody - Anti-c-Fos antibody produced in rabbit
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50-60 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colorectal carcinoma tissue
western blot: 1:1,000 using nuclear extract of a phorbol ester-treated human A431 cells or nuclear extract of human A431 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FOS(2353)

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This Item
SAB5700610SAB4500993SAB4500996
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 50-60 kDa

mol wt

28, 36, 40

mol wt

antigen 40 kDa

mol wt

antigen 40 kDa

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General description

c-Fos belongs to a family of transcription factors, including FosB, Fra1, Fra2. The fos oncogene was isolated as a retroviral transforming gene (v-fos) carried by the FBJ-murine osteosarcoma virus. c-Fos undergoes post-translational modifications and other forms have been described including 57 Da, 60 kDa and 62 kDa proteins. Stable expression of c-Fos has been shown in developing bone tissue and teeth, hematopoietic cells,germ cells, and in the central nervous system. High levels of c-Fos are expressed in human full term fetal membranes.

Immunogen

synthetic peptide corresponding to the N-terminal region of the human c-Fos proto-oncogene (p55), amino acids 3-16.

Application

Rabbit polyclonal anti-c-Fos antibody recognizes c-Fos by immunoblotting, (as single or multiple bands at 50-62 kDa) and by immunohistochemistry. By immunoblotting, the antibody may also detect bands of ~40 kDa representing c-Fos degradation products. Staining of c-Fos by immunoblotting is specifically inhibited with c-Fos immunizing peptide (c-Fos human, amino acids 3-16 with C-terminally added lysine).
Anti-c-Fos antibody produced in rabbit has been used in immunoassays to determine the c-fos immunoreactivity in spinal cord sections. It has been used in Immunohistochemistry.
It may be used as a probe to determine the presence and roles of c-Fos in a wide variety of cell processes including cell proliferation and differentiation, and responses to cell damage or stress.

Biochem/physiol Actions

c-Fos (AP-1 transcription factor subunit) plays an important role in cell proliferation and differentiation. In addition, it is involved in cellular responses to stress, cell damage and death, and has a central role in normal bone and hematopoietic cell development and in oncogenesis. c-Fos is rapidly and transiently induced in almost every cell type upon stimulation by a variety of extracellular stimuli, including stress, mitogenic growth factors, cytokines, neurotransmitters and pharmacological agents. Increased expression of c-Fos is found in some human carcinomas, including colon and pancreatic adenocarcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ying Yang et al.
Nature communications, 7, 11935-11935 (2016-07-15)
Different emotional states lead to distinct behavioural consequences even when faced with the same challenging events. Emotions affect learning and memory capacities, but the underlying neurobiological mechanisms remain elusive. Here we establish models of learned helplessness (LHL) and learned hopefulness
Inhibitory effect of lidocaine on pain and itch using formalin-induced nociception and 5?-guanidinonaltrindole-induced scratching models in mice: behavioral and neuroanatomical evidence.
Inan S, et al.
European Journal of Pharmacology, 616(1-3), 141-146 (2009)
Saadet Inan et al.
European journal of pharmacology, 616(1-3), 141-146 (2009-06-25)
The aim of this study was to establish the effect of lidocaine, a local anesthetic, on pain and itch using formalin-induced nociception and kappa opioid antagonist-induced scratching models in mice. We investigated if local intradermal pretreatment (at -10 min) with
Bone and haematopoietic defects in mice lacking c-fos.
Wang Z Q, et al.
Nature, 360(6406), 741-741 (1992)
Shuo Chen et al.
Nature, 586(7828), 270-274 (2020-10-02)
The ability to recognize information that is incongruous with previous experience is critical for survival. Novelty signals have therefore evolved in the mammalian brain to enhance attention, perception and memory1,2. Although the importance of regions such as the ventral tegmental

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