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ABN26

Sigma-Aldrich

Anti-iNOS/NOS II Antibody, NT

from rabbit, purified by affinity chromatography

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Synonym(s):
Nitric oxide synthase, inducible, Inducible NO synthase, Inducible NOS, iNOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Nos2(18126)

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This Item
SAB4200766MABN527AB5384
Anti-iNOS/NOS II Antibody, NT from rabbit, purified by affinity chromatography

ABN26

Anti-iNOS/NOS II Antibody, NT

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

culture supernatant

antibody form

serum

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

species reactivity

mouse, human

species reactivity

human, mouse, rat, plant

species reactivity

human

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ, immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

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General description

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Specificity

Other homologies: Rat (85% sequence homology).
This antibody recognizes iNOS/NOS II at the N-terminus.

Immunogen

Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II.

Application

Detect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P).
Immunohistochemistry Analysis: 1:50-200 dilution from a representative lot detected iNOS/NOS II in malignant human lung tissues.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated iNOS/NOSII from IFNgamma/LPS treated RAW264.7 cell lysate.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Quality

Evaluated by Western Blot in IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected iNOS/NOS II on 10 µg of IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Target description

~125 kDa observed

Linkage

Replaces: 06-573

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
IFNgamma/LPS untreated and treated RAW264.7 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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