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N7782

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-iNOS
MDL number:
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 130 kDa

species reactivity

rat, mouse

technique(s)

microarray: suitable
western blot: 1:10,000 using cells extract of murine RAW 264.7 macrophages activated with LPS and IFN-γ.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Nos2(18126)
rat ... Nos2(24599)

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N9657SAB4200766N3893
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

biological source

rabbit

biological source

mouse

biological source

mouse

biological source

rabbit

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Gene Information

mouse ... Nos2(18126)
rat ... Nos2(24599)

Gene Information

human ... NOS2(4843)
mouse ... Nos2(18126)
rat ... Nos2(24599)

Gene Information

mouse ... Nos2(18126)

Gene Information

human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)

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General description

Anti-Nitric Oxide Synthase, inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). The NOS isoform characterized in macrophages is a 130 kDa protein, also known as macrophage NOS (macNOS; mNOS), NOS2, or inducible NOS (iNOS). iNOS is an interferon (IFN)-γ-inducible protein. This gene is located on human chromosome 17q11.

Specificity

Anti-Nitric Oxide Synthase, Inducible is specific for nitric oxide synthase (NOS), derived from activated mouse macrophage RAW 264.7 cells (iNOS, 130 kDa), but does not recognize NOS derived from brain tissue (bNOS) and endothelial cells (eNOS). By immunoblotting of an activated mouse macrophage RAW 264.7 cells extract, the staining of the iNOS band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 1126-1144 of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS) conjugated to KLH. The immunogen sequence is highly conserved in rat iNOS and diverges from the human iNOS.

application

Anti-Nitric Oxide Synthase, Inducible antibody produced in rabbit has been used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Inducible nitric oxide synthase (NOS2) produces nitric oxide (NO), which is expected to be lethal to malaria parasites in vitro.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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