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SAB4200766

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Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal

clone NOS-IN, purified from hybridoma cell culture

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Synonym(s):
Anti-Hepatocyte NOS (HEP-NOS), Anti-Inducible NOS (iNOS), Anti-NOS type II, Anti-Peptidyl-cysteine S-nitrosylase NOS2

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NOS-IN, monoclonal

form

buffered aqueous solution

mol wt

~130 kDa

species reactivity

human, mouse, rat, plant

concentration

~1.0 mg/mL

technique(s)

flow cytometry: suitable
immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Nos2(18126)

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1 of 4

This Item
N7782N218N2280
clone

NOS-IN, monoclonal

clone

polyclonal

clone

NOS-3F7-B11-B5, monoclonal

clone

NOS-B1, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

antibody form

ascites fluid

species reactivity

human, mouse, rat, plant

species reactivity

rat, mouse

species reactivity

mouse, bovine, rat

species reactivity

rat, goat, pig, human

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

UniProt accession no.

P35228

UniProt accession no.

P29477

UniProt accession no.

P29476

UniProt accession no.

P29475

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General description

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Inducible-type nitric oxide synthase (NOS) of mouse macrophage origin (iNOS) or macrophage NOS (mNOS) is a type II NOS (NOS2). It is a Ca2+-independent protein, expressed in activated macrophages and some glial cells after stimulation. NOS2 is also present in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. The iNOS gene is mapped to human chromosome 17q11.2. iNOS is a dimeric flavoprotein with a heme group. It comprises the C-terminal reductase domain and an N-terminal oxygenase domain.

Specificity

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal reacts specifically with inducible-type nitric oxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain (bNOS) or with endothelial-type NOS (eNOS). The antibody recognizes iNOS from human, mouse, rat and plant origin.

Immunogen

synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH

Application

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal may be used:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • fluorescence-activated cell sorting (FACS)

Biochem/physiol Actions

Inducible nitric oxide synthase (iNOS) or NOS2 catalyzes the oxidation of L-arginine to L-citrulline and nitric oxide (NO) via an OH-L-arginine intermediate in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen. Elevated levels of iNOS is observed in asthma. A high level of iNOS leads to an increase in NO production which may contribute to cell death and tissue damage. It is also implicated in the pathophysiology of septic shock. Single nucleotide polymorphism in the iNOS gene is indicative of high risk for B and T-cell non-Hodgkin lymphoma. iNOS also elicits macrophage bactericidal functionality.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Christian Bogdan
Trends in immunology, 36(3), 161-178 (2015-02-18)
Thirty years after the discovery of its production by activated macrophages, our appreciation of the diverse roles of nitric oxide (NO) continues to grow. Recent findings have not only expanded our understanding of the mechanisms controlling the expression of NO
iNOS (NOS2) at a glance
Lowenstein CJ and Padalko E
Journal of Cell Science, 117(14), 2865-2867 (2004)
Cleva Villanueva et al.
Free radical biology & medicine, 49(3), 307-316 (2010-04-15)
The effects of nitric oxide in biological systems depend on its steady-state concentration and where it is being produced. The organ where nitric oxide is produced is relevant, and within the organ, which types of cells are actually contributing to
Federica Vannini et al.
Redox biology, 6, 334-343 (2015-09-04)
Nitric oxide (NO) is one of the 10 smallest molecules found in nature. It is a simple gaseous free radical whose predominant functions is that of a messenger through cGMP. In mammals, NO is synthesized by the enzyme nitric oxide
Tadashi Nishiya et al.
The Journal of biological chemistry, 286(11), 9009-9019 (2011-01-05)
Inducible nitric-oxide synthase (iNOS, NOS2) plays a prominent role in macrophage bactericidal and tumoricidal activities. A relatively large amount of NO produced via iNOS, however, also targets the macrophage itself for apoptotic cell death. To uncover the intrinsic mechanisms of

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