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ABN411

Sigma-Aldrich

Anti-Aquaporin-4 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Aquaporin-4, AQP-4, Mercurial-insensitive water channel, MIWC, WCH4
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

technique(s)

western blot: suitable

NCBI accession no.

NP_036957

UniProt accession no.

P47863

shipped in

wet ice

Gene Information

human ... AQP4(361)
mouse ... Aqp4(11829)
rat ... Aqp4(25293)

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This Item
ABN910AB2218MABN2470
Anti-Aquaporin-4 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN411

Anti-Aquaporin-4 Antibody

Anti-Aquaporin 4 from rabbit, purified by affinity chromatography

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ABN910

Anti-Aquaporin 4

Anti-Aquaporin 4 Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB2218

Anti-Aquaporin 4 Antibody

Anti-Aquaporin-4 Antibody, clone mCCD clone mCCD, from mouse

Sigma-Aldrich

MABN2470

Anti-Aquaporin-4 Antibody, clone mCCD

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

mCCD, monoclonal

species reactivity

mouse, rat, human

species reactivity

human, mouse, rat

species reactivity

human, mouse, rat

species reactivity

human, rat, mouse

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_036957

NCBI accession no.

NP_001257487.1

NCBI accession no.

NM_001650.4, NM_004028.3

NCBI accession no.

NP_001641

General description

Aquaporin-4 (AQP-4) is also known as Mercurial-insensitive water channel. AQP-4 is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. It is expressed predominantly in the mature brain, but also in the eye, kidney, intestine and lung. AQP-4 is a multi-pass membrane protein that is part of a complex along with MLC1, TRPV4, HEPACAM and ATP1B1.

Specificity

This antibody recognizes the cytoplasmic domain of Aquaporin-4.

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat Aquaporin-4.

Application

Anti-Aquaporin-4 is an antibody targeting the Aquaporin-4 protein, validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aquaporin-4 in 10 µg of human brain tissue lysate.

Target description

~32 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Yupeng Wang et al.
Frontiers in immunology, 14, 1144532-1144532 (2023-04-15)
Aquaporin-4 immunoglobulin G (AQP4-IgG)-induced astrocytes injury is a key mechanism in the pathogenesis of neuromyelitis spectrum disorder (NMOSD), and although CCL2 is involved, its specific role has not been reported. We aimed to further investigate the role and potential mechanisms
Maria Uldall et al.
Neuroscience letters, 645, 33-39 (2017-02-22)
Acetazolamide is one of the most widely used drugs for lowering intracranial pressure (ICP) and is believed to reduce cerebrospinal fluid (CSF) secretion via its action on the choroid plexus (CP). In the CP the main driving force for CSF
Jacqueline A Hubbard et al.
ASN neuro, 9(1), 1759091416687846-1759091416687846 (2017-01-13)
Maintenance of glutamate and water homeostasis in the brain is crucial to healthy brain activity. Astrocytic glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4) are the main regulators of extracellular glutamate and osmolarity, respectively. Several studies have reported colocalization of GLT1 and
Jacqueline A Hubbard et al.
Experimental neurology, 283(Pt A), 85-96 (2016-05-08)
Astrocytes regulate extracellular glutamate and water homeostasis through the astrocyte-specific membrane proteins glutamate transporter-1 (GLT1) and aquaporin-4 (AQP4), respectively. The role of astrocytes and the regulation of GLT1 and AQP4 in epilepsy are not fully understood. In this study, we
Benjamin J Andreone et al.
Neuron, 94(3), 581-594 (2017-04-19)
The blood-brain barrier (BBB) provides a constant homeostatic brain environment that is essential for proper neural function. An unusually low rate of vesicular transport (transcytosis) has been identified as one of the two unique properties of CNS endothelial cells, relative
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