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MABN2471

Sigma-Aldrich

Anti-Aquaporin-4 Antibody, clone mECD

clone mECD, from mouse

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Synonym(s):
AQP-4, Mercurial-insensitive water channel, MIWC, WCH4
eCl@ss:
32160702

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

mECD, monoclonal

species reactivity

human, rat, mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... AQP4(361)

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This Item
MABN2470AB3594AB2218
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

mECD, monoclonal

clone

mCCD, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, rat, mouse

species reactivity

human, rat, mouse

species reactivity

mouse, human, rat

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

technique(s)

flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

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General description

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NMO) from multiple sclerosis. Clone mECD is shown to induce clustering and internalization of Aquporin-4 and cause its redistribution, which is also observed with NMO-IgG. Exposure to mECD antibody is also shown to cause coaggregation of astrocytic CD28B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl.Acad. Sci. USA 114(21); 5491-5496).

Specificity

Clone mECD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in the extracellular region.

Immunogen

Epitope: extracellular domain
His-tagged full-length human recombinant Aquaporin-4.

Application

Anti-Aquaporin-4, clone mECD, Cat. No. MABN2471, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, and Immunoprecipitation.
Flow Cytometry Analysis: A representative lot detected Aquaporin-4 in Flow Cytometry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

Immunofluorescence Analysis: A representative lot detected Aquaporin-4 in Immunofluorescence applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprcipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci USA. 114(21):5491-5496).
Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry in rat brain frozen tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in Rat brain frozen tissue sections.

Target description

34.83 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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