ABS984

Anti-Intersectin-1 Antibody

serum, from rabbit

• Synonym(s): ITSN-1, ITSN1, Intersectin-1, Intersectin1, ITSN 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_001080955
• UniProt accession no.: O42287
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ITSN1(6453)

Description

General description

Intersectin-1 is a scaffolding protein facilitating clathrin-mediated endocytosis, vesicle trafficking and mitogen-activated protein kinase (MAPK) signaling pathways. Functional association studies of the protein have suggested ITSN1 has several differing roles in various processes such as RAS activation and stimulation of Cdc42 in order to accelerate actin assembly. Alternative splicing produces 4 isoforms. The major isoforms are ITSN1-L and ITSN1-S. Expression for ITSN1 is ubiquitous in adult/fetal tissue, except isoform 1 which is mostly found in the brain. The most notable expression is found in muscle, spleen, skeletal muscle, testis, heart, ovary and all fetal tissue. Additionly, the neurons of young Down Syndrome and pre-Alzheimer’s disease brains are predisposed for enlarged early endosomes which may contribute to these pathological processes.

Immunogen

Epitope: SH3 domain of Xenopus Intersectin-1 protein

GST-tagged recombinant protein corresponding to the SH3 domain of Xenopus Intersectin-1.

Application

Anti-Intersectin-1 is an antibody against Intersectin-1 for use in WB, ICC, IP.

Immunocytochemistry Analysis: A representative lot of this antibody detected Intersectin-1 protein in HEK293T cells (Martin, N., et al. Mol Pharmacol 70:1643–1653, 2006)
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipiated Intersectin-1 (short and long isoforms) from mouse brain lysate (Martin, N., et al. Mol Pharmacol 70:1643–1653, 2006)

Quality

Evaluated by Western Blotting on rat brain cytosol tissue lysate

Western Blotting Analysis: A 1:1000 dilution of this antibody detected Intersectin-1 protein in 10 µg of rat brain cytosol.

Target description

~150 and ~200 kDa observed. Previous publication indicated the 200 kDa to be the long isoform and 150 kDa to be the short isoform (Okur, M., et al. Mol. Cell. Biol. 2014, 34(2):271). Uncharacterized bands may be detected at high antibody concentrations or longer exposures.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1