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ALP10-M

Sigma-Aldrich

Apolipoprotein A-I, human

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Synonym(s):
ApoAI
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

100
300

Assay

>98%

form

liquid

manufacturer/tradename

Chemicon®

NCBI accession no.

UniProt accession no.

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This Item
MSST0001ALP30MABS1310
assay

>98%

assay

≥98% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

-

form

liquid

form

lyophilized powder

form

solid

form

-

NCBI accession no.

NM_000039.1

NCBI accession no.

-

NCBI accession no.

NM_000384.2

NCBI accession no.

NP_000030

UniProt accession no.

P02647

UniProt accession no.

P02647

UniProt accession no.

P04114

UniProt accession no.

P02647

Quality Level

100, 300

Quality Level

200

Quality Level

100

Quality Level

-

General description

High Density Lipoprotein (HDL) isolated by ultracentrifugation are delipidated. Apo A-I is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometric of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with BSA as the standard.

MOLECULAR WEIGHT:

28,000
Product Source: Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.

Physical form

Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.

Storage and Stability

Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-I at +4°C up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Apolipoprotein A-II content of human plasma high density lipoproteins measured by radioimmunoassay.
Schonfeld, G, et al.
Journal of Lipid Research, 18, 645-655 (1977)
Effect of pressure and ionic strength on the self-association of Apo-A-I from the human high density lipoprotein complex.
Formisano, S, et al.
The Journal of Biological Chemistry, 253, 354-359 (1978)
Apolipoprotein A-I enhances activated protein C cytoprotective activity.
Eimear M Gleeson et al.
Blood advances, 4(11), 2404-2408 (2020-06-03)

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