MilliporeSigma
All Photos(1)

Documents

AP112P

Sigma-Aldrich

Goat Anti-Human IgG Antibody, HRP conjugate

Chemicon®, from goat

Sign Into View Organizational & Contract Pricing

eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AP309PAP114PAP120P
antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

isotype

IgG

isotype

IgG

isotype

-

isotype

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It is a glycoprotein and mainly helps in immune defense. About 70 percent of the total immunoglobulin consists of IgG. IgG is a monomeric immunoglobulin, made up of four polypeptide chains. It consists of two 50 kDa heavy chains (gamma) and two 25kDa light chains. These chains are inter-linked by di-sulfide bonds. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Specificity

The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.

Immunogen

Prepared from purified human IgG.

Application

Goat Anti-Human IgG Antibody, HRP conjugate has been used:
  • to detect the bound immunoglobulin G (IgG) in enzyme-linked immunosorbent assay (ELISA) assay
  • in immunohistochemistry or cytochemistry at a dilution of 1:500 - 1:5,000
  • for western blotting with chromogenic substrates at a dilution of 1:5,000 - 1:100,000 and with ECL substrates at a dilution of 1:10,000 - 1:200,000.
  • for EIA at a dilution of 1:5,000-1:100,000

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It initiates the classical pathway of the complement system and also neutralizes virus particles and toxins. Maternal IgG can pass through the human placenta to fetus providing immune defense for the neonate against infections. It facilitates antibody-dependent cell-mediated cytotoxicity (ADCC). IgG is digested by papain to produce fragment antigen-binding (Fab). Pepsin digestion of IgG results in the formation of fragment crystallizable (Fc). IgG levels in severe acute respiratory syndrome coronavirus (SARS-CoV)-2 infected patients may be useful to evaluate the severity and prognosis of coronavirus disease 2019 (COVID-19).

Physical form

Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Other Notes

Research Category: Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Sun Woo Lim et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(10), 10889-10901 (2019-07-04)
Synthetic biologic drugs are highly successful for induction therapy in transplantation, but the development of novel biologics is limited because of the high cost of synthesis and purification. In this study, we developed a novel strategy for the production of
Fulminant Amoebic Colitis: Role of the Innate and Adaptive Immune Responses
Colitis (2012)
Joseph Newman et al.
Nature microbiology, 7(8), 1180-1188 (2022-07-15)
SARS-CoV-2 variants may threaten the effectiveness of vaccines and antivirals to mitigate serious COVID-19 disease. This is of most concern in clinically vulnerable groups such as older adults. We analysed 72 sera samples from 37 individuals, aged 70-89 years, vaccinated with
Arinjay Banerjee et al.
Microorganisms, 8(3) (2020-04-05)
The role of bats in the enzootic cycle of Lyme disease and relapsing fever-causing bacteria is a matter of speculation. In Canada, Borrelia burgdorferi sensu stricto (ss) is the genospecies that is responsible for most cases of Lyme disease in
Kyle E Korolowicz et al.
Cells, 10(9) (2021-09-29)
Current treatment options for patients infected with hepatitis B virus (HBV) are suboptimal, because the approved drugs rarely induce cure due to the persistence of the viral DNA genome in the nucleus of infected hepatocytes, and are associated with either

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service